Gastrointestinal Motility and Improvement Efficacy of Shenhuang Plaster Application on Shenque: Identification, Evaluation, and Mechanism
Gastrointestinal Motility and Improvement Efficacy of Shenhuang Plaster Application on Shenque: Identification, Evaluation, and Mechanism
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DOI：
10.1155/2020/2383970
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发表时间：
2020-07-11
影响因子：
4.1
通讯作者：
Xu, Xiaohong
中科院分区：
文献类型：
--
作者：
Shi, Yanan;Xu, Jingming;Xu, Xiaohong
便秘是紫杉醇（PTX）化疗的副作用之一，是一种胃肠道功能紊乱。在临床环境中，将参黄膏（SHP）贴敷在神阙穴位上可促进肠道运动。在这项研究中，我们阐明了SHP的成分，并评估其对PTX诱导的便秘使用荷瘤小鼠模型的影响。采用传统的中药膏药制备方法制备SHP。使用UPLC-MS/MS分析成分，并通过标准药物数据库中的筛选进行鉴定。通过荧光素标记的葡聚糖在胃肠道中的移动来评价胃肠道转运。苏木精和伊红染色后进行粘膜的组织学研究。使用实时RT-PCR评估mRNA表达，并通过16 s rDNA测序和BLAST分析阐明胎儿微生物群组成。我们的研究结果表明，应用SHP衰减PTX的体重增加抑制，但是，没有观察到对肿瘤生长的抑制作用。PTX诱导的迟缓肠，绒毛，和粘膜基底层的损害显着改善后，应用SHP。此外，SHP增强了PTX对TLR 4及其下游细胞因子以及肠细胞中IL-1 β的刺激效率。SHP与PTX结合重塑了微生物群，这对健康产生了有益的影响。因此，这些结果提供了证据表明，SHP减轻了PTX诱导的便秘和肠道形态学损伤，但增强了PTX对TLR 4通路中细胞因子和IL-1 β表达的影响。因此，我们提出SHP刺激宿主免疫反应以根除癌细胞。
Constipation, a gastrointestinal function disorder, is one of the side effects of paclitaxel (PTX) chemotherapy. Shenhuang plaster (SHP) application on the Shenque acupoint promotes gut motility in clinical settings. In this study, we elucidated the ingredients in SHP and evaluated its effects on PTX-induced constipation using a tumour-bearing mouse model. SHP was prepared using the traditional Chinese plaster preparation method. The ingredients were analysed using UPLC-MS/MS and identified via screening in a standard drug database. The gastrointestinal transit was evaluated by the movement of a fluorescein-labelled dextran in the gastrointestinal tract. A histological study of the mucosa was carried out after haematoxylin and eosin staining. mRNA expression was assessed using real-time RT-PCR, and the foetal microbiota composition was elucidated through 16 s rDNA sequencing and BLAST analysis. Our results indicate that the application of SHP attenuated weight gain inhibition by PTX; however, no inhibitory effect was observed on tumour growth. PTX-induced sluggish intestine, villus, and mucosal base layer damage were significantly improved following the application of SHP. Further, SHP enhanced the stimulation efficiency of PTX on TLR4 and its downstream cytokines, as well as on IL-1 beta in intestinal cells. SHP combined with PTX reshaped the microbiota, which showed beneficial effects on health. Hence, these results provide evidence that SHP alleviates PTX-induced constipation and intestinal morphological damage but augments the effects of PTX on the expression of cytokines in the TLR4 pathway and IL-1 beta. Therefore, we propose that SHP stimulates the host immune response to eradicate cancer cells.