Efficacy of Bifidobacterium breve NCC2950 against DSS-induced colitis is dependent on bacterial preparation and timing of administration
Efficacy of Bifidobacterium breve NCC2950 against DSS-induced colitis is dependent on bacterial preparation and timing of administration
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DOI：
10.3920/bm2013.0039
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发表时间：
2014-03-01
影响因子：
5.4
通讯作者：
Verdu, E. F.
中科院分区：
文献类型：
--
作者：
Hayes, C. L.;Natividad, J. M. M.;Verdu, E. F.
益生菌已被提议作为炎症性肠病的治疗方法，但临床试验中使用的菌株，配方和方案的变化阻碍了其使用指南的制定。因此，对特定益生菌菌株和给药模式的机制的临床前洞察将有助于指导未来的临床试验设计。在本研究中，在结肠炎之前或期间以及在结肠炎再激活之前，将益生菌短双歧杆菌NCC 2950的活的、热灭活（HI）的和用过的培养基制备物给予无特定病原体的C57 BL/6小鼠。使用5天的3.5%葡聚糖硫酸钠饮用水诱导结肠炎。用活性B预处理。Breve降低了疾病的严重程度、髓过氧化物酶活性、显微镜下损伤、细胞因子产生、白细胞介素（IL）-12/IL-10比率和结肠中的淋巴细胞浸润。B。Breve不能减轻正在进行的结肠炎。在急性结肠炎后，使用live和HI B后，疾病症状很快恢复正常。短期治疗;然而，结肠炎的再激活没有得到预防。这些发现表明益生菌调节肠道炎症的功效取决于制剂以及施用时的炎症状态。总的来说，住B。在预防急性结肠炎方面，Breve最为有效。现场直播和嗨B。breve还促进了急性结肠炎后腹泻和结肠出血的恢复。
Probiotics have been proposed as a therapy for inflammatory bowel disease, but variations in strains, formulations, and protocols used in clinical trials have hindered the creation of guidelines for their use. Thus, preclinical insight into the mechanisms of specific probiotic strains and mode of administration would be useful to guide future clinical trial design. In this study, live, heat inactivated (HI), and spent culture medium preparations of the probiotic Bifidobacterium breve NCC2950 were administered to specific pathogen free C57BL/6 mice before or during colitis, as well as before colitis reactivation. Five days of 3.5% dextran sulphate sodium in drinking water was used to induce colitis. Pretreatment with live B. breve reduced disease severity, myeloperoxidase activity, microscopic damage, cytokine production, interleukin (IL)-12/IL-10 ratio, and lymphocyte infiltration in the colon. B. breve did not attenuate on-going colitis. After acute colitis, disease symptoms were normalised sooner with live and HI B. breve treatment; however, reactivation of colitis was not prevented. These findings indicate that the efficacy of a probiotic to modulate intestinal inflammation is dependent on the formulation as well as state of inflammation when administered. Overall, live B. breve was most efficacious in preventing acute colitis. Live and HI B. breve also promoted recovery from diarrhoea and colon bleeding after a bout of acute colitis.