Arabidopsis ABI5 plays a role in regulating ROS homeostasis by activating CATALASE 1 transcription in seed germination.
Arabidopsis ABI5 plays a role in regulating ROS homeostasis by activating CATALASE 1 transcription in seed germination.
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拟南芥 ABI5 通过激活种子萌发中的 CATALASE 1 转录来调节 ROS 稳态
DOI：
10.1007/s11103-017-0603-y
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发表时间：
2017-05
影响因子：
5.1
通讯作者：
Wang XF
中科院分区：
文献类型：
--
作者：
Bi C;Ma Y;Wu Z;Yu YT;Liang S;Lu K;Wang XF
ABA不敏感基因5 （ABA INSENSITIVE 5, ABI5）在调控种子萌发中起着至关重要的作用。在本研究中，我们发现用3-氨基-1,2,4-三唑（3-AT）抑制过氧化氢酶活性可以抑制Col-0、abi5突变体和abi5过表达转基因系的种子萌发。与Col-0相比，abi5突变体种子在萌发过程中对3-AT更加敏感，而abi5过表达转基因株系种子则表现出不敏感。H2O2对Col-0、abi5突变体和abi5过表达转基因株系种子萌发的影响与3-AT相同。这些结果表明，ROS参与了ABI5介导的种子萌发过程。此外，我们观察到拟南芥中三种过氧化氢酶成员的T-DNA插入突变体在种子萌发过程中表现出3- at不敏感或-超敏感的表型，这表明这些过氧化氢酶成员以高度复杂的方式调节ROS稳态。ABI5通过影响过氧化氢酶的表达和活性来影响活性氧（ROS）稳态。此外，我们发现ABI5直接结合到CAT1启动子并激活CAT1表达。遗传证据支持CAT1在种子萌发过程中发挥ABI5下游ROS信号传导作用的观点。rna测序分析表明，abi5-1种子中参与ROS代谢过程的基因或应答ROS胁迫的基因转录受损。此外，由于ABI5在3-AT处理下的表达变化，一些与种子萌发相关的基因的表达也发生了变化。总之，所有的研究结果表明，ABI5至少部分通过影响活性氧稳态来调节种子萌发。
It has been known that ABA INSENSITIVE 5 (ABI5) plays a vital role in regulating seed germination. In the present study, we showed that inhibition of the catalase activity with 3-amino-1,2,4-triazole (3-AT) inhibits seed germination of Col-0, abi5 mutants and ABI5-overexpression transgenic lines. Compared with Col-0, the seeds of abi5 mutants showed more sensitive to 3-AT during seed germination, while the seeds of ABI5-overexpression transgenic lines showed more insensitive. H2O2 showed the same effect on seed germination of Col-0, abi5 mutants and ABI5-overexpression transgenic lines as 3-AT. These results suggest that ROS is involved in the seed germination mediated by ABI5. Further, we observed that T-DNA insertion mutants of the three catalase members in Arabidopsis displayed 3-AT-insensitive or -hypersensitive phenotypes during seed germination, suggesting that these catalase members regulate ROS homeostasis in a highly complex way. ABI5 affects reactive oxygen species (ROS) homeostasis by affecting CATALASE expression and catalase activity. Furthermore, we showed that ABI5 directly binds to the CAT1 promoter and activates CAT1 expression. Genetic evidence supports the idea that CAT1 functions downstream of ABI5 in ROS signaling during seed germination. RNA-sequencing analysis indicates that the transcription of the genes involved in ROS metabolic process or genes responsive to ROS stress is impaired in abi5-1 seeds. Additionally, expression changes in some genes correlative to seed germination were showed due to the change in ABI5 expression under 3-AT treatment. Together, all the findings suggest that ABI5 regulates seed germination at least partly by affecting ROS homeostasis.