BDNF and pro-BDNF in serum and exosomes in major depression: Evolution after antidepressant treatment
BDNF and pro-BDNF in serum and exosomes in major depression: Evolution after antidepressant treatment
复制标题
DOI：
10.1016/j.pnpbp.2020.110229
复制
发表时间：
2021-07-13
影响因子：
5.6
通讯作者：
Girard, Murielle
中科院分区：
文献类型：
--
作者：
Gelle, Thibaut;Samey, Rayhanatou Altine;Girard, Murielle
背景：重性抑郁症临床相关生物学指标的研究具有重要意义。脑源性神经营养因子（BDNF）通过其神经营养作用在MD中发挥重要作用，其水平显著降低。其前体proBDNF具有相反的作用，其血清水平的变化尚不清楚。它们在血清和外泌体之间的分布及其在抗抑郁治疗期间的演变也尚不清楚，可能在调节其作用方面很重要。本研究的目的是评估与对照组相比，MD患者在抗抑郁治疗期间血清和外泌体mBDNF和proBDNF水平是否发生改变，以及它们与临床改善和临床变量的关系。材料和方法：纳入42例MD患者和40例对照。在抗抑郁药物治疗开始后3周和7周的3次就诊期间，收集了评估抑郁和认知障碍严重程度的问卷和血液样本。ELISA法检测血清和外泌体中mBDNF和proBDNF水平。结果：与对照组相比，MD受试者在D0时血清和外泌体BDNF水平降低，proBDNF水平升高。在抗抑郁治疗期间，血清和外泌体中BDNF和前BDNF呈反比变化。BDNF和proBDNF水平与临床改善和抑郁量表无关系。结论：我们证明了这些分子在MD治疗后在血清或外泌体中发生了进化。这些转运囊泡可能在BDNF的调节中起作用。
Background: The study of clinically related biological indicators in Major Depression (MD) is important. The Brain Derived Neurotrophic Factor (BDNF) appears to play an important role in MD, through its neurotrophic effect, and its levels are significantly decreased. The variation in the serum levels of its precursor proBDNF, which has opposite effects, is not known. Their distribution between serum and exosomes and their evolution during antidepressant treatment is also not known, and may be important in modulating their effects.The aim of this study is to evaluate whether serum and exosome mBDNF and proBDNF levels are altered in patients with MD during antidepressant treatment compared to controls, and their association with clinical improvement and clinical variables.Materials and methods: 42 MD subjects and 40 controls were included. Questionnaires to assess the severity of depression and cognitive impairment and blood samples were collected during the three visits at D0 (inclusion) and 3 and 7 weeks after the start of antidepressant treatment. Assays for mBDNF and proBDNF levels were performed in serum and exosomes by ELISA.Results: MD subjects had decreased serum and exosomal BDNF levels and increased proBDNF levels at D0 compared to controls. BDNF and pro-BDNF vary in an inverse manner in both serum and exosomes during antidepressant treatment. No relationship of BDNF and proBDNF levels to clinical improvement and depression scales was found.Conclusion: We demonstrated an evolution of those molecules either in serum or in exosomes after MD treatment. These transport vesicles could have a role in the regulation of BDNF.