Cis-acting sequences which regulate expression of the Sgs-4 glue protein gene of Drosophila.
Cis-acting sequences which regulate expression of the Sgs-4 glue protein gene of Drosophila.
复制标题
调节果蝇 Sgs-4 胶蛋白基因表达的顺式作用序列。
DOI：
10.1002/j.1460-2075.1986.tb04501.x
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Beckendorf,SK
中科院分区：
文献类型：
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作者：
McNabb,SL;Beckendorf,SK
果蝇的Sgs-4胶蛋白基因仅在三龄幼虫唾液腺中表达。以前的工作表明，调控区位于5′端，远离基因，如组织特异性DNA酶I超敏区域和超敏区域DNA损伤的突变体产生不足所示。在这里，我们通过对含有Sgs-4的克隆片段进行种系转化证明，该基因5′端840 bp和3′端130 bp之间的序列足以产生Sgs-4活性。当5′序列被移除到-392时，活性被消除，从而验证了上游必需序列的存在。除了正常表达水平的能力外，有活性的片段还包括其他三种顺式作用调节活性：发育时间、组织特异性和剂量补偿。相比之下，测试的碎片没有说明通常与Sgs-4相关的粉扑的形成。如染色体重排所示，膨化所需的区域限于基因周围的16 - 19 kb。
The Sgs‐4 glue protein gene of Drosophila is expressed only in third‐instar larval salivary glands. Previous work suggests that a regulatory region lies 5′ and remote to the gene, as indicated by a region of tissue‐specific DNase I hypersensitivity and by underproducing mutants with DNA lesions in the hypersensitive region. Here we demonstrate by germ line transformation of cloned fragments containing Sgs‐4 that the sequences between 840 bp 5′ and 130 bp 3′ to the gene are sufficient for Sgs‐4 activity. When 5′ sequence was removed to ‐392, activity was eliminated, thereby verifying the existence of essential sequences far upstream. Fragments that are active include, in addition to the capacity for normal levels of expression, three other cis‐acting regulatory activities: developmental timing, tissue specificity, and dosage compensation. In contrast, the fragments tested did not specify formation of the puff with which Sgs‐4 is normally associated. As shown by chromosomal rearrangements, the region required for puffing is limited to 16‐19 kb surrounding the gene.