DNA Mechanics Affected by Small DNA Interacting Ligands
DNA Mechanics Affected by Small DNA Interacting Ligands
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DOI：
10.1002/1438-5171(200206)3:2/3
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发表时间：
2002-06
期刊：
影响因子：
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通讯作者：
Sudhir Husale;W. Grange;M. Hegner
中科院分区：
文献类型：
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作者：
Sudhir Husale;W. Grange;M. Hegner
我们已经研究了暴露于DNA结合配体的单个DNA链的力学。通过光镊测量的这些试剂与单个dsDNA链的相互作用清楚地表明配体-DNA结合模式。如预期的，如果化合物是嵌入的，则检测到轮廓长度的增加。沟结合剂影响先前“裸”dsDNA链的过度拉伸能力。我们使SYBR®绿色I与裸dsDNA相互作用。用于核酸凝胶染色的该化合物的结合模式尚不清楚。SYBR®的相互作用机制通过光镊实验揭示。单双链DNA片段的力延伸曲线显示出沟结合模式，这不影响分子的轮廓长度，但显着改变了双链DNA的过度拉伸行为。
We have investigated the mechanics of individual DNA strands exposed to DNA binding ligands. The interaction of these agents with individual dsDNA strands measured by optical tweezers clearly indicates the ligand-DNA binding mode. As expected, if the compound is intercalating then an increase of contour length is detected. Groove binders affect the overstretching capabilities of the formerly “naked” dsDNA strand. We interacted SYBR®Green I with naked dsDNA. The binding mode of this compound, which is used for nucleic acid gel staining, is not known. The mechanics of the interaction of SYBR® is revealed by optical tweezers experiments. The force extension curves on single dsDNA fragments show a groove-binding mode, which does not affect the contour length of the molecule but significantly alters the overstretching behaviour of the dsDNA.