Liquid-chromatographic determination of isoenzymes of alkaline phosphatase in serum and tissue homogenates.
Liquid-chromatographic determination of isoenzymes of alkaline phosphatase in serum and tissue homogenates.
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DOI：
10.1093/clinchem/32.5.816
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发表时间：
1986-05
影响因子：
9.3
通讯作者：
E. Schoenau;K. Herzog;H. Boehles
中科院分区：
文献类型：
--
作者：
E. Schoenau;K. Herzog;H. Boehles
我们描述了一种通过“高效”液相色谱法分离碱性磷酸酶（AP）同工酶的新方法。用LiCl的逐步梯度从柱（Mono Q HR 5/5，强阴离子交换剂）洗脱同工酶。通过组织匀浆、病理血清和热灭活鉴定了来自小肠、骨、肝和胆汁的同工酶。
We describe a new method for separating alkaline phosphatase (AP) isoenzymes by means of "high-performance" liquid chromatography. Isoenzymes are eluted from the column (Mono Q HR 5/5, a strong anion-exchanger) with a stepwise gradient of LiCl. The isoenzymes originating from small intestine, bone, liver, and bile were identified by use of tissue homogenates, pathological sera, and heat inactivation.