Human fetal globin DNA sequences suggest novel conversion event.
Human fetal globin DNA sequences suggest novel conversion event.
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人类胎儿珠蛋白 DNA 序列表明存在新的转化事件。
DOI：
10.1093/nar/12.11.4469
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发表时间：
1984
影响因子：
14.9
通讯作者：
Weissman,SM
中科院分区：
文献类型：
--
作者：
StoeckertJr,CJ;Collins,FS;Weissman,SM
对最近克隆的两个人类Aγ球蛋白等位基因进行了测序研究，发现了一些碱基差异，它们聚集在大内含子(IVS-2)上。一个等位基因具有先前未描述的IVS-2序列。大多数等位基因差异可以解释为Gγ作为供体的基因转换事件的结果。这一事件的一个新特点是在Aγ基因的未转换区域中穿插了三个Cγ-HKE区域。我们认为，位于两个转化点之间的嘌呤-嘧啶交替序列是转化事件的起始点。与基因转化模型一致，S1-核酸酶检测表明，这种多(嘌呤-嘧啶)链在超螺旋载体中具有单链特征。
DNA sequencing studies of two recently cloned human Aγ globln alleles has revealed a number of base differences which are clustered in the large intron (IVS-2). One allele has a previously undescribed IVS-2 sequence. Most of the allelic differences can be explained as resulting from a gene conversion event involving Gγ as a donor. A novel feature of this event is that three Cγ-Hke regions occur interspersed among unconverted areas of the Aγgene. We propose that an alternating purine-pyrimidine run which is located between two of the converted sites is the initiation site of the conversion event. Consistent with models of gene conversion, this poly (purine-pyrimidine) tract has single-stranded characteristics in supercoiled plasmids as assayed by S1-nuclease.