RNAither, an automated pipeline for the statistical analysis of high-throughput RNAi screens
RNAither, an automated pipeline for the statistical analysis of high-throughput RNAi screens
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DOI：
10.1093/bioinformatics/btp014
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发表时间：
2009-03-01
期刊：
影响因子：
5.8
通讯作者：
Kaderali, Lars
中科院分区：
文献类型：
--
作者：
Rieber, Nora;Knapp, Bettina;Kaderali, Lars
我们提出了RNAither，一个免费的统计环境R的软件包，它执行高通量RNA干扰（RNAi）敲低实验的分析，从原始实验数据中生成相关基因和途径的列表。该文库提供了信号强度的质量评估，以及广泛的数据标准化选项，用于鉴定显著siRNA的不同统计学检验，以及涉及相应基因的生物过程的显著性分析。分析结果以一组HTML页面的形式呈现。此外，所有值和图都可以作为文本文件或pdf和png文件提供。
We present RNAither, a package for the free statistical environment R which performs an analysis of high-throughput RNA interference (RNAi) knock-down experiments, generating lists of relevant genes and pathways out of raw experimental data. The library provides a quality assessment of the signal intensities, as well as a broad range of options for data normalization, different statistical tests for the identification of significant siRNAs, and a significance analysis of the biological processes involving corresponding genes. The results of the analysis are presented as a set of HTML pages. Additionally, all values and plots are available as either text files or pdf and png files.