Effect of pore velocity on biodegradation of cis-dichloroethene (DCE) in column experiments.
Effect of pore velocity on biodegradation of cis-dichloroethene (DCE) in column experiments.
复制标题
柱实验中孔速对顺式二氯乙烯 (DCE) 生物降解的影响。
DOI：
10.1007/s10532-009-9307-6
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发表时间：
2010
期刊：
影响因子：
3.6
通讯作者：
Cunningham,JeffreyA
中科院分区：
文献类型：
--
作者：
Mendoza-Sanchez,Itza;Autenrieth,RobinL;McDonald,ThomasJ;Cunningham,JeffreyA
进行柱实验来评估孔速对顺式二氯乙烯（cis-DCE）在多孔介质中运输过程中生物降解程度的影响。柱子填充均匀的玻璃珠，并接种能够将四氯乙烯完全脱氯为乙烯的培养物。塔进水中保持恒定浓度的cis-DCE。一式两份测试三种不同的孔速度，使每个柱承受恒定的速度。在高流速下，顺式-DCE 几乎在柱的停留时间内完全降解为乙烯。然而，在中等和低流速下，观察到脱氯不完全。 7 周后，从柱中收集 DNA 以确定微生物种群的差异。结果表明，Dehalococcoidessp。高速柱中存在的量较高，与观察到的脱氯一致。这些结果表明，在受污染的地下水位点，地下水流速的异质性可能是导致生物活性异质分布的因素之一。
Column experiments were conducted to evaluate the effect of pore velocity on the extent of biodegradation ofcis-dichloroethene (cis-DCE) during transport in porous media. Columns were filled with homogeneous glass beads and inoculated with a culture capable of complete dechlorination of tetrachloroethene to ethene. A constant concentration ofcis-DCE was maintained in the columns’ influent. Three different pore velocities were tested in duplicate, subjecting each column to a constant velocity. At high flow velocity, degradation ofcis-DCE to ethene was nearly complete within the residence time of the columns. However, at medium and low flow velocities, incomplete dechlorination was observed. After 7 weeks, DNA was harvested from the columns to determine differences in the microbial populations. Results suggest thatDehalococcoidessp. were present in higher quantities in the high-velocity columns, consistent with the observed dechlorination. These results suggest that, at contaminated groundwater sites, heterogeneity of groundwater velocity may be one factor that contributes to heterogeneous distribution of biological activity.