Transfer RNA modifications that alter +1 frameshifting in general fail to affect -1 frameshifting.
Transfer RNA modifications that alter +1 frameshifting in general fail to affect -1 frameshifting.
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DOI：
10.1261/rna.5210803
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发表时间：
2003-06
期刊：
影响因子：
4.5
通讯作者：
Jaunius Urbonavičius;G. Stahl;J. Durand;S. B. Ben Salem;Qiang Qian;P. Farabaugh;G. Björk
中科院分区：
文献类型：
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作者：
Jaunius Urbonavičius;G. Stahl;J. Durand;S. B. Ben Salem;Qiang Qian;P. Farabaugh;G. Björk
利用变种人（tgt，mnmA（asuE，trmU），mnmE（trmE），miaA，miaB，miaE，truA（hisT），truB）和酿酒酵母的trm 5突变体，分析了修饰核苷Q34，mnm（5）s（2）U34，ms（2）io（6）A37，Psi 39，Psi 55，m（1）G37，和yW 37在各种七聚体序列上的-1移码错误，在这些七聚体序列上至少一个密码子被具有这些修饰的核苷的tRNA解码。-1移码的频率在同类菌株中是相同的，仅不同的是各种tRNA修饰基因的等位基因状态。事实上，在一种情况下（mnm（5）s（2）U34缺陷），我们观察到修饰不足的tRNA产生-1移码错误的能力降低。这些结果与早期的观察形成鲜明对比，即tRNA修饰阻止+1移码，表明-1和+1移码错误发生的机制不同。解释这些结果的可能机制进行了讨论。
Using mutants (tgt, mnmA(asuE, trmU), mnmE(trmE), miaA, miaB, miaE, truA(hisT), truB) of either Escherichia coli or Salmonella enterica serovar Typhimurium and the trm5 mutant of Saccharomyces cerevisiae, we have analyzed the influence by the modified nucleosides Q34, mnm(5)s(2)U34, ms(2)io(6)A37, Psi39, Psi55, m(1)G37, and yW37 on -1 frameshifts errors at various heptameric sequences, at which at least one codon is decoded by tRNAs having these modified nucleosides. The frequency of -1 frameshifting was the same in congenic strains only differing in the allelic state of the various tRNA modification genes. In fact, in one case (deficiency of mnm(5)s(2)U34), we observed a reduced ability of the undermodified tRNA to make a -1 frameshift error. These results are in sharp contrast to earlier observations that tRNA modification prevents +1 frameshifting suggesting that the mechanisms by which -1 and +1 frameshift errors occur are different. Possible mechanisms explaining these results are discussed.