Effects of Zinc Alpha2 Glycoprotein on Lipid Metabolism of Liver in High-Fat Diet-Induced Obese Mice
Effects of Zinc Alpha2 Glycoprotein on Lipid Metabolism of Liver in High-Fat Diet-Induced Obese Mice
复制标题
锌α2糖蛋白对高脂饮食诱导肥胖小鼠肝脏脂质代谢的影响
DOI：
10.1055/s-0043-118910
复制
发表时间：
2017-10-01
影响因子：
2.2
通讯作者：
Yang, Xiaojing
中科院分区：
文献类型：
--
作者：
Fan, Guoqiang;Qiao, Yu;Yang, Xiaojing
摘要锌α2糖蛋白是一种参与脂肪组织动员的新型脂肪因子，但对其在肝脏中的脂代谢作用知之甚少。因此，我们研究了ZAG在调节肝脏脂质蓄积中的作用。将小鼠随机分为两组，一组喂普通饲料，另一组喂高脂饲料8周，建立肥胖模型。然后，将正常饮食组分为注射pcDNA3.1的ND组和注射ZAG重组质粒的NDZ组，将高脂饮食组分为注射pcDNA3.1的HF组和注射ZAG重组质粒的HFZ组。每周称一次体重，每三天注射一次，共8次。结果表明，与HF组相比，HFZ组大鼠体重和肝脏TG含量均显著降低。HFZ组硬脂酰辅酶A合成酶1(SCD1)和酰辅酶A合成酶1(ACSS1)蛋白水平显著降低。此外，HFZ组的磷酸化激素敏感脂肪酶(P-HSL)水平明显升高。HFZ组大鼠肝脏脂肪酸转位酶(CD36)和脂肪酸结合蛋白-1(FABP1)蛋白水平降低。此外，HFZ组磷酸化蛋白激酶A(PPKA)的表达高于HF组。同时，与ND组相比，NDZ组大鼠体重明显减轻，P-HSL水平显著升高，肝脏TG含量无明显变化。因此，ZAG可能有利于预防高脂饮食所致的肝脂代谢紊乱。
Abstract Zinc alpha2 glycoprotein (ZAG) is a new type of adipokine involved in adipose tissue mobilization, however, little is known about its lipid metabolism effect in liver. Therefore, we investigated the effects of ZAG in the regulation of hepatic lipid accumulation. Mice were randomly divided into two groups; one was fed a normal diet and another was fed a high-fat diet for eight weeks to establish obesity model. After that, the normal diet group was divided into ND (injection of pcDNA3.1) and NDZ (injection of ZAG recombinant plasmid) and the high-fat diet group was divided into HF (injection of pcDNA3.1) and HFZ (injection of ZAG recombinant plasmid). The mice were weighed once per week and injected with plasmid once every three days for eight times. The results showed that body weight and hepatic TG content were decreased dramatically in HFZ group compared with HF group. The stearoyl-CoAdesaturase1 (SCD1) and Acyl-CoA Synthetase-1 (ACSS1) protein levels in HFZ group were significantly decreased. Furthermore, phosphorylated hormone sensitive lipase (P-HSL) was significantly higher in HFZ group. In HFZ group, hepatic fatty acid translocase (CD36) and fatty acids binding protein-1 (FABP1) protein levels were reduced. In addition, the expression of phosphorylated protein kinase A (PPKA) in HFZ group was higher than the HF group. Meanwhile, NDZ group showed significantly decreased body weight and increased P-HSL level though the hepatic TG content showed no significantly changes compared with the ND group. Therefore, we conclude that ZAG may be beneficial for preventing high-fat-diet-induced hepatic lipid metabolic disorders.