CELLULAR PROTEINS REACTIVE WITH MONOCLONAL-ANTIBODIES DIRECTED AGAINST SIMIAN VIRUS-40 T-ANTIGEN
CELLULAR PROTEINS REACTIVE WITH MONOCLONAL-ANTIBODIES DIRECTED AGAINST SIMIAN VIRUS-40 T-ANTIGEN
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DOI：
10.1128/jvi.42.2.612-620.1982
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发表时间：
1982-01-01
影响因子：
5.4
通讯作者：
HARLOW, E
中科院分区：
文献类型：
--
作者：
CRAWFORD, L;LEPPARD, K;HARLOW, E
最近分离的几种单克隆抗体与SV 40 T抗原反应，也与未感染和未转化细胞中发现的蛋白质反应。这些蛋白质彼此不同，PAb 419与35，000 MW蛋白质反应，PAb 427与75，000 MW磷蛋白反应，PAb 405与150，000 MW磷蛋白反应，PAb 204与68，000 MW蛋白质反应。尽管这些交叉反应中的一些可能是偶然的，但作为替代，它们可能反映了病毒T抗原和相关宿主蛋白的结构域之间的形状和功能的相似性。[本研究中使用了非洲绿色猴肾CV1、小鼠3 TG、BALB/c 3 T3 A31和瑞士3 T3 K、Potorous tridactylus PTK 1和PTK 2、人宫颈肿瘤C33-I、乳腺癌肉瘤Hs 578 T、淋巴母细胞样Namalwa和成纤维细胞。]
Several recently isolated monoclonal antibodies which reacted with SV40 T antigens also reacted with proteins found in uninfected and untransformed cells. The proteins were different from each other, PAb419 reacting with a 35,000 MW protein, PAb427 reacting with a 75,000 MW phosphoprotein, PAb405 reacting with a 150,000 MW phosphoprotein and PAb204 reacting with a 68,000 MW protein. Although some of these cross-reactions may be fortuitous, they may, as an alternative, reflect similarities of shape and perhaps function between domains of the viral T antigen and the relevant host proteins. [African green monkey kidney CV1, mouse 3TG, BALB/c 3T3 A31 and swiss 3T3 K, Potorous tridactylus PTK1 and PTK2, human cervical tumor C33-I, mammary carcinosarcoma Hs578T, lymphoblastoid Namalwa and fibroblast cells were used in this study.].