SHEDDING OF GS PROTEIN (A SOLUBLE FORM OF THE VIRAL GLYCOPROTEIN) BY THE RABIES VIRUS-INFECTED BHK-21-CELLS
SHEDDING OF GS PROTEIN (A SOLUBLE FORM OF THE VIRAL GLYCOPROTEIN) BY THE RABIES VIRUS-INFECTED BHK-21-CELLS
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DOI：
10.1006/viro.1993.1405
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发表时间：
1993-08-01
期刊：
影响因子：
3.7
通讯作者：
KAWAI, A
中科院分区：
文献类型：
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作者：
MORIMOTO, K;IWATANI, Y;KAWAI, A
我们研究了狂犬病毒感染BHK-21细胞培养物中G蛋白脱落的可能机制。定量分析表明，约10%的新合成的G蛋白作为Gs蛋白从细胞脱落到培养基中，Gs蛋白是G蛋白的可溶形式，其缺乏蛋白的C-末端锚定区域。脉冲追踪实验表明，氚标记的Gs蛋白的释放开始后不久的合成，但停止后很短的时间（约2小时内）。另一方面，病毒粒子相关的G蛋白的释放开始后30至60分钟开始的Gs蛋白脱落，并持续超过10 hr. Gs蛋白不能检测到在细胞中的生化和免疫学方法的时候，大量的Gs蛋白被积极脱落。用抗G蛋白C-末端的抗血清，我们可以在细胞中检测到一种多肽，其大小似乎与Gs蛋白丢失的多肽相同。这些结果表明，Gs蛋白是由新合成的G蛋白的蛋白水解切割产生的，并且切割似乎在G蛋白成熟的某个阶段停止。
We investigated a possible mechanism of G protein shedding occurring in the rabies virus-infected BHK-21 cell cultures. Quantitative analysis showed that about 10% of the newly synthesized G proteins were shed from the cells as Gs protein, a soluble form of G protein which lacked the C-terminal anchoring region of the protein, into the culture medium. Pulse-chase experiments revealed that the release of tritium-labeled Gs proteins began soon after the synthesis, but ceased after a short time (within about 2 hr). On the other hand, the release of the virion-associated G protein began 30 to 60 min behind the start of Gs protein shedding and continued for more than 10 hr. Gs protein could not be detected in the cells by biochemical and immunological methods at the time when large amounts of Gs protein were actively being shed. With an antiserum against the C-terminal of G protein we could detect in the cell a polypeptide having a size that is seemingly the same as that which was lost from Gs protein. These results suggest that Gs proteins are generated by a proteolytic cleavage of newly synthesized G proteins, and the cleavage seems to be stopped at a certain stage of the G protein maturation.