In vitro expression of the endothelial phenotype:: Comparative study of primary isolated cells and cell lines, including the novel cell line HPMEC-ST1.6R
In vitro expression of the endothelial phenotype:: Comparative study of primary isolated cells and cell lines, including the novel cell line HPMEC-ST1.6R
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DOI：
10.1006/mvre.2002.2434
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发表时间：
2002-11-01
影响因子：
3.1
通讯作者：
Kirkpatrick, CJ
中科院分区：
文献类型：
--
作者：
Unger, RE;Krump-Konvalinkova, V;Kirkpatrick, CJ
内皮细胞系通常用于体外研究以避免与使用原代内皮细胞相关的问题，例如存在污染细胞、难以获得大量细胞以及在体外增殖过程中细胞活力和内皮标志物表达的逐渐丧失。我们分析了细胞系EA.hy926、ECV 304、EVLC 2、HAEND、HMEC-1、ISOHAS-1和我们实验室最近产生的细胞系HPMEC-ST 1. 6 R中定义不同内皮细胞表型的特征，并将这些表型与从脐静脉（HUVEC）、肺（HPMEC）和皮肤（HDMEC）分离的原代人内皮细胞中发现的表型进行了比较。分析揭示了原代细胞和细胞系之间表型表达的显著差异。在原代细胞中通常观察到血管性血友病因子、CD 31和CD 34的组成型表达，以及促炎刺激物刺激时细胞粘附分子、ICAM-1、VCAM-1和E-选择素和细胞因子、IL-6、IL-8、MCP-1和GM-CSF的诱导表达，以及DiI-Ac-LDL的摄取和Matrigel上索状结构的形成。然而，大多数细胞系仅表现出少数这些内皮特征。只有HPMEC-ST 1. 6 R表现出主要的组成型和诱导型内皮细胞特征，并在Matrigel上显示出与原代HPMEC相似的血管生成反应。因此，HPMEC-ST 1. 6 R将是一个有价值的体外模型系统，在体外研究的病理机制和成熟的微血管内皮细胞的血管生成。(C)2002 Elsevier Science（美国）。
Endothelial cell lines are commonly used in in vitro studies to avoid problems associated with the use of primary endothelial cells such as the presence of contaminating cells, the difficulty in obtaining larger numbers of cells, as well as the progressive loss of cell viability and expression of endothelial markers in the course of in vitro propagation. We have analyzed the characteristics defining distinctive enclothelial phenotypes in the cell lines EA.hy926, ECV304, EVLC2, HAEND, HMEC-1, ISOHAS-1 and a cell line recently generated in our laboratory, HPMEC-ST1.6R, and have compared these phenotypes with those found in primary human endothelial cells isolated from umbilical vein (HUVEC), lung (HPMEC), and skin (HDMEC). The analysis revealed significant differences in phenotype expression between primary cells and the cell lines. Constitutive expression of von Willebrand factor, CD31, and CD34 and induced expression of cell adhesion molecules, ICAM-1, VCAM-1, and E-selectin and cytokines, IL-6, IL-8, MCP-1, and GM-CSF on stimulation with proinflammatory stimuli, as well as the uptake of DiI-Ac-LDL and the formation of cord-like structures on Matrigel, were typically observed in the primary cells. However, most cell lines exhibited only a few of these endothelial characteristics. Only HPMEC-ST1.6R exhibited the major constitutive and inducible endothelial cell characteristics and showed an angiogenic response on Matrigel similar to that of primary HPMEC. Thus, HPMEC-ST1.6R will be a valuable in vitro model system in which to study pathomechanisms and angiogenesis of the mature microvascular endothelium in vitro. (C) 2002 Elsevier Science (USA).