The synthesis of citrate-modified silver nanoparticles in an aqueous suspension of graphene oxide nanosheets and their antibacterial activity
The synthesis of citrate-modified silver nanoparticles in an aqueous suspension of graphene oxide nanosheets and their antibacterial activity
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DOI：
10.1016/j.colsurfb.2012.12.033
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发表时间：
2013-05-01
影响因子：
5.8
通讯作者：
Boukherroub, R.
中科院分区：
文献类型：
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作者：
Das, M. R.;Sarma, R. K.;Boukherroub, R.
由沉积在氧化石墨烯（GO）纳米片上的银纳米颗粒（Ag NP）组成的复合材料通过在柠檬酸三钠的存在下用硼氢化钠（NaBH 4）化学还原Ag金属离子来制备，柠檬酸三钠用作稳定剂以防止纳米颗粒的团聚。采用紫外可见光谱（UV/vis）、X射线光电子能谱（XPS）、X射线衍射（XRD）和透射电子显微镜（TEM）对GO/Ag纳米粒子复合材料进行了表征。TEM分析证实了GO纳米片上具有高密度的Ag NP，粒度范围为2 - 25 nm。研究了GO/Ag NPs悬浮液作为抗革兰氏阳性细菌金黄色葡萄球菌和枯草芽孢杆菌的抗菌剂的活性。发现Ag NP（不含GO）杀死细菌菌落的百分比为96 - 97%，而仅使用GO/Ag NP悬浮液获得100%杀死细菌菌落。此外，还观察到糖和蛋白质从两种S. aureus和B.枯草芽孢杆菌与GO/Ag NP悬浮液的相互作用中的pH值高于Ag NP（不含GO）和GO纳米片。
A composite material consisting of silver nanoparticles (Ag NPs) deposited on graphene oxide (GO) nanosheets is prepared by chemical reduction of Ag metal ions by sodium borohydride (NaBH4) in the presence of trisodium citrate acting as a stabilizing agent to prevent agglomeration of the nanoparticles. The synthesized GO/Ag NPs composite was characterized by UV/vis spectroscopy, X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and transmission electron microscopy (TEM). TEM analysis confirmed a high density of Ag NPs on the GO nanosheets with a particle size range of 2–25nm. The activity of the GO/Ag NPs suspension as an antibacterial agent against Gram positive bacteria Staphylococcus aureus and Bacillus subtilis was investigated. The percentage of the killing bacterial colonies by Ag NPs (without GO) is found to be 96–97% while 100% of killing bacterial colonies is only obtained using GO/Ag NPs suspension. Moreover, it was also observed that leakage of sugars and proteins from the cell wall of both S. aureus and B. subtilis in interaction with GO/Ag NPs suspension is higher compared to Ag NPs (without GO) and GO nanosheets.