Controlled Release of Basic Fibroblast Growth Factor From Gelatin Hydrogel Sheet Improves Structural and Physiological Properties of Vein Graft in Rat
Controlled Release of Basic Fibroblast Growth Factor From Gelatin Hydrogel Sheet Improves Structural and Physiological Properties of Vein Graft in Rat
复制标题
从明胶水凝胶片中控制释放碱性成纤维细胞生长因子可改善大鼠静脉移植物的结构和生理特性
DOI：
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Y. Okita
中科院分区：
文献类型：
--
作者：
Tomonori Haraguchi;K. Okada;Y. Tabata;Y. Maniwa;Y. Hayashi;Y. Okita
目的-自体静脉移植物仍被广泛使用，但其长期通畅性不佳。本研究的目的是确定是否包裹静脉移植明胶水凝胶片纳入碱性成纤维细胞生长因子改善其机械和生理特性。方法和结果：将大鼠自体股静脉插入腹主动脉。将大鼠分为3组：未处理的移植物（A组）、包裹在无碱性成纤维细胞生长因子的明胶水凝胶片中的移植物（B组）和包裹在碱性成纤维细胞生长因子浸渍的明胶水凝胶片中的移植物（C组）。第1天，A组可见内皮细胞脱落，A、B组中膜破裂，末端脱氧核苷酸转移酶介导的dUTP缺口末端标记染色阳性。与此相反，在C组中的媒体保持完整和末端脱氧核苷酸转移酶介导的dUTP缺口末端标记阴性，与MAPK的激活。与A组相比，B和C组移植物扩张明显受到抑制，C组新生内膜增殖程度最小。8周时，C组移植物中出现具有均匀弹性层的新生内膜，存在显示新生血管的刚性新生外膜，并且血流速度最高。结论：用碱性成纤维细胞生长因子浸渍的明胶水凝胶片包裹静脉移植物改善了它们的结构和生理特性，因此也可能改善长期通畅性。
Objectives—Autologous vein grafts are still widely used, but their long-term patency is suboptimal. The objective of the current study was to determine whether wrapping a vein graft in gelatin hydrogel sheet incorporating basic fibroblast growth factor improves their mechanical and physiological properties. Methods and Results—Autologous femoral vein was interposed into the abdominal aorta in rats. The rats were divided into 3 groups: nontreated grafts (group A), grafts wrapped in basic fibroblast growth factor-free gelatin hydrogel sheet (group B), and grafts wrapped in basic fibroblast growth factor-impregnated gelatin hydrogel sheet (group C). On day 1, endothelial desquamation was observed in group A, and the media in groups A and B were disrupted, staining positive in the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assay. In contrast, the media in group C remained intact and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling–negative, associated with activation of MAPK. Graft dilation was significantly inhibited in groups B and C compared with group A, with those in group C showing the smallest degree of neointimal proliferation. At 8 weeks grafts in group C developed neointima with homogeneous elastic laminae, presence of rigid neoadventitia that displayed neovascularity, and the highest blood flow velocity. Conclusions—Wrapping vein grafts in basic fibroblast growth factor- impregnated gelatin hydrogel sheet improved their structural and physiological properties, and might therefore also improve long-term patency.
DOI：
10.1152/ajpheart.1995.269.5.h1641
发表时间：
1995-11-01
影响因子：
4.8
作者：
CROWLEY, ST;RAY, CJ;HORWITZ, LD
通讯作者：
HORWITZ, LD
影响因子：
4.3
作者：
Yamashita, A;Hanna, AK;Sumpio, BE
通讯作者：
Sumpio, BE