An integrated needle-type biosensor for intravascular glucose and lactate monitoring
An integrated needle-type biosensor for intravascular glucose and lactate monitoring
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DOI：
10.1002/(sici)1521-4109(199809)10:11
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发表时间：
1998-09-01
期刊：
影响因子：
3
通讯作者：
Wilkins, E
中科院分区：
文献类型：
--
作者：
Yang, QL;Atanasov, P;Wilkins, E
一种用于血管内乳酸和葡萄糖同时监测的针式集成生物传感器被开发出来。为了使整个传感器小型化并集成到皮下注射针头中，葡萄糖传感器的工作电极采用在针头表面电沉积铂，然后电聚合1,3-苯二胺作为内层的方法来消除可氧化生理物质的干扰，并稳定铂颗粒。乳酸传感器的工作电极由铂丝制成，铂丝固定在针空心体中。以戊二醛为交联剂，二硫苏糖醇为稳定剂，固定化葡萄糖氧化酶和乳酸氧化酶。外扩散控制膜采用PVC涂层。在体外对传感器的性能进行了评估，该传感器对乳酸的灵敏度为10 nA/mM，对葡萄糖的灵敏度为7.5 nA/mM，对乳酸的灵敏度为16 mM，对葡萄糖的灵敏度为24 mM。对传感器在血浆中的响应评价显示出与在缓冲溶液中观察到的相似的灵敏度和线性范围。两个单独的传感器对乳酸和葡萄糖都有特异性反应，没有观察到显著的交叉反应性。传感器在4℃的缓冲液中保存3个月以上，其性能变化不大。
A needle-type integrated biosensor has been developed for simultaneous intravascular lactate and glucose monitoring. In order to miniaturize the whole sensor and incorporate it into a hypodermic needle, the working electrode of the glucose sensor was made by electrodeposition of platinum on the needle surface followed by the electropolymerization of 1,3-phenylenediaminr as the inner layer to eliminate the interference from oxidizable physiological substances and to stabilize the platinum particles. The working electrode of lactate sensor was made from platinum wire which was fixed in the needle hollow body. Enzymes (glucose oxidase and lactate oxidase) were immobilized with glutaraldehyde as cross-linking reagent and dithiothreitol as the stabilizer. PVC coating was used as the external diffusion control membrane. Sensor performance was evaluated in vitro and the sensor shows a sensitivity of 10 nA/mM for lactate, and 7.5 nA/mM for glucose, and a linear I ang of up to 16 mM for lactate and 24 mM for glucose. Evaluation of the sensor response in blood plasma showed similar sensitivity and linear range as observed in buffer solution. Both individual sensors reacted specifically to lactate and glucose and no cross-reactivity of significance was observed. Sensors showed a mi ni mum change in their performance when stored in buffer solution at 4 degrees C for more than 3 months.