A host basal transcription factor is a key component for infection of rice by TALE-carrying bacteria
A host basal transcription factor is a key component for infection of rice by TALE-carrying bacteria
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宿主基础转录因子是携带 TALE 的细菌感染水稻的关键成分
DOI：
10.7554/elife.19605
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发表时间：
2016-07-29
期刊：
影响因子：
7.7
通讯作者：
Wang, Shiping
中科院分区：
文献类型：
--
作者：
Yuan, Meng;Ke, Yinggen;Wang, Shiping
类转录激活因子效应子 (TALE) 是在一系列植物病原细菌中发现的序列特异性 DNA 结合蛋白，它们在宿主与病原体的相互作用中发挥着重要作用。然而，目前尚不清楚 TALE 在被注射到宿主细胞中后如何激活感染成功所需的宿主基因的转录。在这里，我们证明来自水稻的基础转录因子 IIA γ 亚基 TFIIAγ5 是携带 TALE 的细菌 Xanthomonas oryzae pv. 感染的关键成分。米曲霉，白叶枯病的病原体。疾病易感基因的激活需要多个 TALE 与 TFIIAγ5 的直接相互作用。相反，TFIIAγ5宿主基因表达的减少限制了易感基因的诱导，从而减少白叶枯病症状。 TFIIAγ5 的抑制或突变还可以减少细菌条纹病，这是另一种由携带 TALE 的稻瘟病菌引起的毁灭性水稻病害。稻科拉。这些结果对于制定广泛适用的策略来提高植物对携带 TALE 的病原体的抗性具有重要意义。 DOI：http://dx.doi.org/10.7554/eLife.19605.001
Transcription activator-like effectors (TALEs) are sequence-specific DNA binding proteins found in a range of plant pathogenic bacteria, where they play important roles in host-pathogen interactions. However, it has been unclear how TALEs, after they have been injected into the host cells, activate transcription of host genes required for infection success. Here, we show that the basal transcription factor IIA gamma subunit TFIIAγ5 from rice is a key component for infection by the TALE-carrying bacterium Xanthomonas oryzae pv. oryzae, the causal agent for bacterial blight. Direct interaction of several TALEs with TFIIAγ5 is required for activation of disease susceptibility genes. Conversely, reduced expression of the TFIIAγ5 host gene limits the induction of susceptibility genes and thus decreases bacterial blight symptoms. Suppression or mutation of TFIIAγ5 can also reduce bacterial streak, another devastating disease of rice caused by TALE-carrying X. oryzae pv. oryzicola. These results have important implications for formulating a widely applicable strategy with which to improve resistance of plants to TALE-carrying pathogens. DOI: http://dx.doi.org/10.7554/eLife.19605.001