INSERTION OF N REGIONS INTO HEAVY-CHAIN GENES IS CORRELATED WITH EXPRESSION OF TERMINAL DEOXYTRANSFERASE IN B-CELLS
INSERTION OF N REGIONS INTO HEAVY-CHAIN GENES IS CORRELATED WITH EXPRESSION OF TERMINAL DEOXYTRANSFERASE IN B-CELLS
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DOI：
10.1038/311752a0
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发表时间：
1984-01-01
期刊：
影响因子：
64.8
通讯作者：
BALTIMORE, D
中科院分区：
文献类型：
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作者：
DESIDERIO, SV;YANCOPOULOS, GD;BALTIMORE, D
免疫球蛋白重链的可变区在种系中由三个离散的DNA片段编码：VH（可变）元件、D（多样性）元件和jh（连接）元件。在B淋巴细胞分化过程中，来自每一组的单个片段通过重组聚集在一起，形成完整的vhdjhvariable区1 - 8。为了更好地理解这些过程，我们现在分离并测序了两种细胞系中重链基因重排的中间产物（DJHfusions）和最终产物（VH-to-DJHjoins）的分子克隆，这两种细胞系代表了b淋巴细胞分化早期阶段的类似细胞11 - 13。重链基因在一个细胞系中组装，而在另一个细胞系中不组装，这伴随着在重组连接处出现短核苷酸插入。这些插入的产生与这些细胞系中末端脱氧核苷酸转移酶的表达呈正相关。
The variable regions of immunoglobulin heavy chains are encoded in the germ line by three discrete DNA segments:VH(variable) elements,D(diversity) elements andJH(joining) elements. During the differentiation of B lymphocytes, individual segments from each group are brought together by recombination to form the completeVHDJHvariable region1–8. To understand these processes better, we have now isolated and sequenced molecular clones representing intermediates (DJHfusions) and final products (VH-to-DJHjoins) of heavy-chain gene rearrangement in two cell lines9,10that represent analogues of cells at early stages of B-lymphocyte differentiation11–13. Heavy-chain gene assembly in one cell line but not in the other is accompanied by the appearance of short nucleotide insertions at the recombinational junctions. The generation of such insertions is positively correlated with the expression of terminal deoxynucleotidyl transferase in these lines.