NITRIC-OXIDE AND PROSTACYCLIN - DIVERGENCE OF INHIBITORY EFFECTS ON MONOCYTE CHEMOTAXIS AND ADHESION TO ENDOTHELIUM INVITRO
NITRIC-OXIDE AND PROSTACYCLIN - DIVERGENCE OF INHIBITORY EFFECTS ON MONOCYTE CHEMOTAXIS AND ADHESION TO ENDOTHELIUM INVITRO
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DOI：
10.1161/01.atv.11.2.254
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发表时间：
1991-03-01
期刊：
影响因子：
--
通讯作者：
MARTIN, JF
中科院分区：
文献类型：
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作者：
BATH, PMW;HASSALL, DG;MARTIN, JF
单核细胞与内皮细胞的相互作用在决定单核细胞从血流进入血管壁的运动中起着至关重要的作用。本研究报告了两种内皮衍生因子，一氧化氮和前列环素，改变体外单核细胞的行为。一氧化氮（bbb10 (-5) M）抑制单核细胞粘附于猪主动脉内皮细胞单层，而丙环素(10（-9)~ 10(-5)M）没有作用。一氧化氮和前列环素均能抑制n -甲酰基-蛋氨酸-酰基-苯丙氨酸刺激的单核细胞趋化性，并分别诱导细胞内环单磷酸鸟苷和环单磷酸腺苷浓度的剂量依赖性增加。CD11b/CD18粘附受体（一种已知介导单核细胞间粘附的糖蛋白复合物）的细胞表面表达未被一氧化氮或前列环素改变。因此，内皮衍生的一氧化氮和前列环素可能在调节单核细胞-血管壁相互作用中具有生理作用。该系统的改变可能导致单核细胞从血流向血管壁的迁移增加，从而导致动脉粥样硬化。
Monocyte-endothelial interactions are of fundamental importance in determining the movement of monocytes from the blood stream into the vessel wall. This study reports that two endothelium-derived factors, nitric oxide and prostacyclin, alter in vitro monocyte behavior. Nitric oxide (> 10(-5) M) inhibited monocyte adhesion to porcine aortic endothelial cell monolayers, whereas prostacyclin (10(-9) to 10(-5) M) had no effect. Both nitric oxide and prostacyclin inhibited monocyte chemotaxis stimulated by N-formyl-methionyl-leucyl-phenylalanine and induced dose-dependent increases in intracellular cyclic guanosine monophosphate and cyclic adenosine monophosphate concentrations, respectively. The cell surface expression of the CD11b/CD18 adhesion receptor, a glycoprotein complex known to mediate monocyte intercellular adhesion, was not altered by either nitric oxide or by prostacyclin. Thus, endothelium-derived nitric oxide and prostacyclin may have a physiological role in modulating monocyte-vascular wall interactions. Alterations in this system may contribute to the increased monocyte emigration from the blood stream into the vessel wall observed in atherogenesis.