Increased cationic fluxes in stimulated lymphocytes of the mouse: response of enriched B- and T-cell subpopulations to B- and T-cell mitogens.
Increased cationic fluxes in stimulated lymphocytes of the mouse: response of enriched B- and T-cell subpopulations to B- and T-cell mitogens.
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小鼠受刺激淋巴细胞中的阳离子通量增加：富集的 B 细胞和 T 细胞亚群对 B 细胞和 T 细胞有丝分裂原的反应。
DOI：
10.1139/o80-116
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
J. Kaplan
中科院分区：
文献类型：
--
作者：
T. Owens;J. Kaplan
小鼠(Balb/c)脾淋巴细胞悬液在有丝分裂原刀豆蛋白A(ConA)刺激前1小时摄取钾的净速率(K+)为4.6 ± 0.2 fmolcell−1，刺激后1 h摄取率为8.5 ± 0.4 fmolcell−−1。这些净转运速率与之前报道的静息和刺激的人外周血淋巴细胞的净转运速率相似。老鼠系统和人类系统的一个不同之处是，后者在刺激后几分钟内注意到的转运增加，在前者变得明显之前需要6 h。当使用纯合子、先天无配子的脾淋巴细胞进行类似的转运研究时。裸小鼠ConA是一种T细胞有丝分裂原，既不引起原始反应(操作上定义为在有丝裂原存在的情况下培养48h后标记胸苷摄取到DNA中的增加)，也不引起阳离子内流增加；B细胞有丝分裂原脂多糖(LPS)既不引起转移率的增加，也不引起阳离子内流的增加。
Suspensions of mouse (Balb/c) splenic lymphocytes took up the potassium congener 86Rb at a net rate (calculated as K+) of 4.6 ± 0.2 fmol cell−1 h−1 before stimulation with the mitogen concanavalin A (Con A) and of 8.5 ± 0.4 fmol cell−1 h−1 after stimulation. These net transport rates were of similar magnitude to those previously reported in the case of resting and stimulated human peripheral blood lymphocytes. One difference between the mouse and human systems was the increased transport noted within minutes of stimulation in the case of the latter that required 6 h before becoming evident in the former. When similar transport studies were performed using splenic lymphocytes of the homozygous, congenitally athymic. nude mouse, Con A, a T-cell mitogen, caused neither a blastogenic response (operationally defined as increased uptake of labeled thymidine into DNA after 48-h culture in presence of mitogen) nor the increase in cation influx; lipopolysaccharide (LPS), a B-cell mitogen, caused both increased tra...