Gaba, in some cases together with glycine, is used as the inhibitory transmitter by pump cells in the hering-breuer reflex pathway of the rat
Gaba, in some cases together with glycine, is used as the inhibitory transmitter by pump cells in the hering-breuer reflex pathway of the rat
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DOI：
10.1016/j.neuroscience.2004.05.032
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发表时间：
2004-01-01
期刊：
影响因子：
3.3
通讯作者：
Tanaka, I
中科院分区：
文献类型：
--
作者：
Ezure, K;Tanaka, I
Hering-Breuer 反射是基本呼吸反射之一，由缓慢适应的肺牵张感受器的二阶中继神经元介导。这些神经元被称为泵细胞，位于孤束核中，包括一群抑制性神经元。我们的目的是确定抑制性泵细胞使用哪种递质，GABA 还是甘氨酸。此外，我们还检查了快速适应肺牵张受体（RAR 细胞）的二级中继神经元（其兴奋性或抑制性性质尚不清楚）是否使用这些抑制性神经递质。在戊巴比妥麻醉、神经肌肉阻断和人工通气的大鼠中，我们用神经生物素标记泵细胞 (n = 33) 和 RAR 细胞 (n = 26)，并使用原位处理组织以检测编码谷氨酸脱羧酶亚型 67 (GAD67) 或甘氨酸转运蛋白 2 (GLYT2) 的 mRNA 杂交。泵细胞位于间质核及其附近，RAR 细胞位于连合亚核。大多数 (64%) 检查 GAD67 mRNA 的泵细胞和许多 (26%) 检查 GLYT2 mRNA 的泵细胞表达各自的 mRNA。在两种 mRNA 均被双重检测的 8 个泵细胞中，3 个表达两种 mRNA，1 个表达 GAD67 mRNA 但不表达 GLYT2 mRNA，另外 4 个不表达 mRNA。另一方面，RAR 细胞既不表达 GAD67 mRNA 也不表达 GLYT2 mRNA。结果表明，抑制性泵细胞基本上是 GABA 能的，其中一些可能同时释放 GABA 和甘氨酸，而 RAR 细胞既不是 GABA 能的，也不是甘氨酸能的。这些发现扩展了我们对肺受体介导的反射网络（包括 Hering-Breuer 反射）的理解。 (C) 2004 年国际广播组织。由爱思唯尔有限公司出版。保留所有权利。
The Hering-Breuer reflex is one of the fundamental respiratory reflexes and is mediated by second-order relay neurons of the slowly adapting lung stretch receptors. These neurons, which are called pump cells, are located in the nucleus tractus solitarii and include a population of inhibitory neurons. We aimed to determine which transmitter, GABA or glycine, the inhibitory pump cells use. In addition, we examined whether or not second-order relay neurons of the rapidly-adapting lung stretch receptors (RAR-cells), whose excitatory or inhibitory nature is not known, use these inhibitory neurotransmitters. In Nembutal-anesthetized, neuromuscularly blocked and artificially ventilated rats, we labeled pump cells (n=33) and RAR-cells (n=26) with Neurobiotin and processed the tissues for detection of mRNA encoding either glutamic acid decarboxylase isoform 67 (GAD67) or glycine transporter 2 (GLYT2) using in situ hybridization. The pump cells were located in the interstitial nucleus and its vicinity and the RAR-cells in the commissural subnucleus. The majority (64%) of the pump cells examined for GAD67 mRNA and many (26%) of the pump cells examined for GLYT2 mRNA expressed respective mRNAs. Of the eight pump cells in which both mRNAs were double-detected, three expressed both mRNAs and one expressed GAD67 mRNA but not GLYT2 mRNA, the other four expressing neither mRNAs. On the other hand, RAR-cells expressed neither GAD67 mRNA nor GLYT2 mRNA. The results suggest that the inhibitory pump cells are basically GABAergic and some of them may corelease GABA and glycine, and that RAR-cells are neither GABAergic nor glycinergic. These findings expand our understanding of the networks of lung receptor-mediated reflexes including the Hering-Breuer reflex. (C) 2004 IBRO. Published by Elsevier Ltd. All rights reserved.