2D Z-scheme ZnIn2S4/g-C3N4 heterojunction based on photoelectrochemical immunosensor with enhanced carrier separation for sensitive detection of CEA.
2D Z-scheme ZnIn2S4/g-C3N4 heterojunction based on photoelectrochemical immunosensor with enhanced carrier separation for sensitive detection of CEA.
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DOI：
10.1016/j.bios.2023.115926
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发表时间：
2023-12
影响因子：
12.6
通讯作者：
Yiran Bo;Linrong Li;Pei Miao;Chengfang Li;Jing Chang;Yang Zhang;Yanfeng Lv;Xiaofeng Yang
中科院分区：
文献类型：
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作者：
Yiran Bo;Linrong Li;Pei Miao;Chengfang Li;Jing Chang;Yang Zhang;Yanfeng Lv;Xiaofeng Yang
基于光电化学（PEC）传感器的半导体材料已被广泛用于检测。同时，低效率的载流子分离限制了PEC传感器的灵敏度。因此，基于二维Z型ZnIn 2S 4/g-C3 N4异质结作为光敏材料，BiVO 4作为光猝灭剂，设计了一种新型的光电化学免疫传感器，用于癌胚抗原（CEA）的灵敏检测。首先，二维ZnIn 2S 4/g-C3 N4结构提供了大量的活化位点，这有利于捕获抗体（Ab 1）的负载。其次，Z型异质结具有高的氧化还原容量，同时促进光生电子-空穴对（e−/h+）的快速分离和迁移。因此，它能够在一定程度上消耗更多的电子供体，导致更高的初始光电流。此外，首次引入了具有较大空间电位电阻的BiVO 4，实现了信号放大。BiVO 4不仅可以与衬底材料竞争电子供体，而且可以有效地阻止电子供体与衬底接触，进一步降低光电流信号。在优化条件下，该传感器对CEA的检测范围为0.0001 ~ 100 ng/mL，检测下限为0.03 pg/mL。该传感器特异性强、稳定性好、重现性好，为构建准确、方便的PEC免疫传感器用于生物分析和疾病早期诊断提供了新的思路。
Semiconducting materials based on photoelectrochemical (PEC) sensors have been widely utilized for detection. Meanwhile, the sensitivity of the PEC sensor was limited by low-efficiency carrier separation. Thus, a novel sandwich-type PEC bioimmunosensing based on 2D Z-scheme ZnIn2S4/g-C3N4heterojunction as a photosensitive material and BiVO4as a photoquencher was designed for the sensitive detection of carcinoembryonic antigen (CEA). Firstly, the 2D ZnIn2S4/g-C3N4structure provided a multitude of activated sites which facilitated the loading of the capture antibody (Ab1). Secondly, the Z-scheme heterojunction had a high redox capacity while promoting the rapid separation and migration of photogenerated electron-hole pairs (e−/h+). Thus it was able to consume more electron donors to a certain extent, resulting in a higher initial photocurrent. In addition, BiVO4with large spatial potential resistance was introduced for the first time to realize signal amplification. BiVO4could not only compete with substrate materials for electron donors, but also effectively prevent electron donors from contacting the substrate, further reducing the photocurrent signal. Under optimized conditions, the sensor had a favorable detection range (0.0001–100 ng/mL) to CEA and a low detection limit of 0.03 pg/mL. With high specificity, excellent stability, and remarkable reproducibility, this sensor provided a new perspective for constructing accurate and convenient PEC immunosensor for bioanalysis and early disease diagnosisdisease diagnosis.