Molecular evolution of functional nucleic acids with chemical modifications.
Molecular evolution of functional nucleic acids with chemical modifications.
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DOI：
10.3390/molecules15085423
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发表时间：
2010-08-09
期刊：
影响因子：
--
通讯作者：
Sugimoto N
中科院分区：
文献类型：
--
作者：
Kuwahara M;Sugimoto N
核酸是创造功能分子的有吸引力的材料，可以用作催化剂、特定粘合剂和分子开关。具有这种功能的核酸可以通过随机筛选获得，通常使用体外选择方法。这些方法有助于探索核酸的潜在能力，并稳步促进其进化，即创造RNA/DNA酶，适体和适体酶。化学改性将是进一步提高其性能的关键手段，如扩大功能多样性、增强活性和改善生物稳定性。的确，在过去的二十年中，涉及化学修饰、后selex化学修饰和合理设计的随机筛选方法已经取得了进展，这些方法的结合和整合可能会产生一类新的功能核酸。本文综述了化学修饰对核酸功能分子进化的影响，并对相关技术进行了展望。
Nucleic acids are attractive materials for creating functional molecules that have applications as catalysts, specific binders, and molecular switches. Nucleic acids having such functions can be obtained by random screening, typically using in vitro selection methods. These methods have helped explore the potential abilities of nucleic acids and steadily contributed to their evolution, i.e., creation of RNA/DNA enzymes, aptamers, and aptazymes. Chemical modification would be a key means to further increase their performance, e.g., expansion of function diversity, enhancement of activity, and improvement of biostability for biological use. Indeed, in the past two decades, random screening involving chemical modification, post-SELEX chemical modification, and rational design methods have been advanced, and combining and integrating these methods may produce a new class of functional nucleic acids. This review focuses on the effectiveness of chemical modifications on the evolution of nucleic acids as functional molecules and the outlook for related technologies.
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影响因子：
15
作者：
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通讯作者：
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DOI：
10.1046/j.1432-1327.2000.01557.x
发表时间：
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期刊：
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