High-grain feeding causes strong shifts in ruminal epithelial bacterial community and expression of Toll-like receptor genes in goats.
High-grain feeding causes strong shifts in ruminal epithelial bacterial community and expression of Toll-like receptor genes in goats.
复制标题
高谷物喂养导致山羊瘤胃上皮细菌群落和 Toll 样受体基因表达的强烈变化
DOI：
10.3389/fmicb.2015.00167
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发表时间：
2015
影响因子：
5.2
通讯作者：
Mao SY
中科院分区：
文献类型：
--
作者：
Liu JH;Bian GR;Zhu WY;Mao SY
集约化山羊生产中使用的高谷物 (HG) 饲喂会影响瘤胃壁的生理机能，但壁外细菌群落和宿主 Toll 样受体 (TLR) 所引起的变化尚不清楚。在这项研究中，10 只雄性山羊被随机分配到两组，分别饲喂干草饲料（0% 谷物；n= 5）或 HG 饲料（65% 谷物；n= 5）。使用焦磷酸测序和定量实时聚合酶链反应测定长期（7周）HG喂养期间瘤胃上皮细菌群落和TLR表达的变化。主坐标分析和分子方差分析（AMOVA）结果表明，HG 喂养引起细菌组成和结构的强烈变化。在属水平上，我们的数据显示，它增加了类群Butyrivibrio、未分类的梭状芽胞杆菌、莫吉杆菌、未分类的Anaerolineaceae和Succiniclasticum的相对丰度，并降低了未分类的瘤胃球菌科、未分类的Rikenellaceae、未分类的丹毒菌科、Howardella和未分类的Neisseriaceae的比例。 HG喂养的山羊还表现出瘤胃上皮中TLR2、TLR3和TLR5的相对mRNA表达上调(P<0.05)。相关分析显示，TLR表达量的增加与瘤胃上皮细菌相对丰度的变化有关。这项研究首次深入了解了山羊瘤胃上皮细菌群体对 HG 喂养的适应性反应，并表明这些变化与 TLR 表达的变化相关。这些发现为理解反刍动物宿主-微生物关系提供了新的见解。
High-grain (HG) feeding used in intensive goat production can affect the physiology of the rumen wall, but the changes induced in the epimural bacterial community and host Toll-like receptors (TLRs) are not well understood. In this study, 10 male goats were randomly allocated to two groups and fed either a hay diet (0% grain;n= 5) or an HG diet (65% grain;n= 5). The changes in the ruminal epithelial bacterial community and expression of TLRs during long-term (7 weeks) HG feeding were determined using pyrosequencing and quantitative real-time polymerase chain reaction. Principal coordinate analysis and analysis of molecular variance (AMOVA) results showed that HG feeding caused a strong shift in bacterial composition and structure. At the genus level, our data revealed that it increased the relative abundance of taxaButyrivibrio, unclassifiedClostridiales,Mogibacterium, unclassifiedAnaerolineaceae, andSucciniclasticum, and decreased the proportion of unclassifiedRuminococcaceae, unclassifiedRikenellaceae, unclassifiedErysipelotrichaceae,Howardella, and unclassifiedNeisseriaceae. The HG-fed goats also exhibited upregulation of the relative mRNA expression of TLR2, TLR3, and TLR5 in the rumen epithelium (P< 0.05). Correlation analysis revealed that the increase in TLR expression was associated with changes in the relative abundance of ruminal epithelial bacteria. This study provides a first insight into the adaptive response of ruminal epithelial bacterial populations to HG feeding in goats and shows that these changes were associated with alterations in TLR expression. These findings provide new insight into understanding of host–microbial relationships in ruminants.