Radiation-sensitive mutants of Caenorhabditis elegans.
Radiation-sensitive mutants of Caenorhabditis elegans.
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DOI：
10.1093/genetics/102.2.159
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发表时间：
1982-10
期刊：
影响因子：
3.3
通讯作者：
P. Hartman;R. K. Herman
中科院分区：
文献类型：
--
作者：
P. Hartman;R. K. Herman
从秀丽隐杆线虫（Caenorhabditis elegans）中分离出9个对紫外线敏感的rad（异常辐射敏感）突变体。这些突变对它们的野生型等位基因是隐性的，映射到线虫的6个连锁群中的4个，并定义了9个新的游戏，命名为rad-1到rad-9。其中两种突变体——rad-1和rad-2——对X射线非常敏感，另外三种——rad-2、rad-3和rad-4——在特定的暴露条件下对甲磺酸甲酯过敏。这些突变体对一种以上DNA损伤剂的超敏反应表明，它们可能在DNA修复方面存在异常。一种突变体——rad-5，一种温度敏感的不育突变体——在多个位点显示出更高的自发突变频率；rad-4表现出冷敏感的胚胎发生，将减数分裂x染色体不分离性降低10倍，并部分抑制一些但不是全部增加减数分裂x染色体不分离性的突变；在25度时，拉德-6雌雄同体的生存能力是拉德-6雄性的一半；新成熟的（但不是老的）rad-8雌雄同体产生许多不能存活的胚胎后代。对7个rad突变体的减数分裂重组频率进行了测量，发现减数分裂重组频率接近正常。
Nine rad (for abnormal radiation sensitivity) mutants hypersensitive to ultraviolet light were isolated in the small nematode Caenorhabditis elegans. The mutations are recessive to their wild-type alleles, map to four of the six linkage groups in C. elegans and define nine new games named rad-1 through rad-9. Two of the mutants--rad-1 and rad-2--are very hypersensitive to X rays, and three--rad-2, rad-3 and rad-4--are hypersensitive to methyl methanesulfonate under particular conditions of exposure. The hypersensitivity of these mutants to more than one DNA-damaging agent suggests that they may be abnormal in DNA repair. One mutant--rad-5, a temperature-sensitive sterile mutant--shows an elevated frequency of spontaneous mutation at more than one locus; rad-4, which shows a cold-sensitive embryogenesis, reduces meiotic X-chromosome nondisjunction tenfold and partially suppresses some but not all mutations that increase meiotic X-chromosome nondisjunction; the viability of rad-6 hermaphrodites is half that of rad-6 males at 25 degrees; and newly mature (but not older) rad-8 hermaphrodites produce many inviable embryo progeny. Meiotic recombination frequencies were measured for seven rad mutants and found to be close to normal.