Specialized cells tag sexual and species identity in Drosophila melanogaster
Specialized cells tag sexual and species identity in Drosophila melanogaster
复制标题
DOI：
10.1038/nature08495
复制
发表时间：
2009-10-15
期刊：
影响因子：
64.8
通讯作者：
Levine, Joel D.
中科院分区：
文献类型：
--
作者：
Billeter, Jean-Christophe;Atallah, Jade;Levine, Joel D.
社会互动依赖于个体之间的相互识别，在这种情况下，许多生物体使用化学信号来指示物种和性别。表皮碳氢化合物信号被昆虫（包括果蝇）用来区分同种个体和其他个体（1-3）。这些化学物质也有助于种内求偶和交配的相互作用（1-3）。然而，性别和物种识别是由共同的化学信号机制联系在一起的可能性尚未得到正式测试。在这里，我们提供了直接的证据表明，一个单一的化合物是用来沟通女性身份之间的D。melanogaster之间的生殖隔离屏障。黑腹菌和兄弟种。转基因操作消除表皮碳氢化合物通过消融的卵母细胞，专门的细胞需要表达这些化学信号。由此产生的无酒细胞（oe（-））雌性会引发雄性的正常求爱行为，但实际上求爱雄性比野生型雌性更喜欢。此外，野生型雄性试图与oe（-）雄性交配。因此，缺乏碳氢化合物的果蝇是一种性刺激过度。与野生型雌性相比，用令人厌恶的雄性信息素顺式乙酸乙烯酯（cVA）处理处女雌性显著延迟了oe（-）雌性的交配。当用cVA和雌性催情剂（7Z，11 Z）-二十七碳二烯（7，11-HD）的混合物处理oe（-）雌性时，这种差异被消除，表明雌性催情剂化合物可以减弱雄性厌恶信息素的作用。7，11-HD也被证明在异种相遇中具有关键作用。特别是，由于其他果蝇属的雄性向oe（-）D求爱，物种屏障消失了。melanogaster雌虫，D. simulans雄性一直与它们交配。用7，11-HD处理oe（-）雌性动物恢复了物种屏障，表明单一化合物可以赋予物种身份。这些结果确定了一个共同的机制，性和物种识别表皮碳氢化合物调节。
Social interactions depend on individuals recognizing each other, and in this context many organisms use chemical signals to indicate species and sex(1). Cuticular hydrocarbon signals are used by insects, including Drosophila melanogaster, to distinguish conspecific individuals from others(1-3). These chemicals also contribute to intraspecific courtship and mating interactions(1-3). However, the possibility that sex and species identification are linked by common chemical signalling mechanisms has not been formally tested. Here we provide direct evidence that a single compound is used to communicate female identity among D. melanogaster, and to define a reproductive isolation barrier between D. melanogaster and sibling species. A transgenic manipulation eliminated cuticular hydrocarbons by ablating the oenocytes, specialized cells required for the expression of these chemical signals. The resulting oenocyte-less (oe(-)) females elicited the normal repertoire of courtship behaviours from males, but were actually preferred over wild-type females by courting males. In addition, wild-type males attempted to copulate with oe(-) males. Thus, flies lacking hydrocarbons are a sexual hyperstimulus. Treatment of virgin females with the aversive male pheromone cis-vaccenyl acetate (cVA) significantly delayed mating of oe(-) females compared to wild-type females. This difference was eliminated when oe(-) females were treated with a blend of cVA and the female aphrodisiac (7Z,11Z)-heptacosadiene (7,11-HD), showing that female aphrodisiac compounds can attenuate the effects of male aversive pheromones. 7,11-HD also was shown to have a crucial role in heterospecific encounters. Specifically, the species barrier was lost because males of other Drosophila species courted oe(-) D. melanogaster females, and D. simulans males consistently mated with them. Treatment of oe(-) females with 7,11-HD restored the species barrier, showing that a single compound can confer species identity. These results identify a common mechanism for sexual and species recognition regulated by cuticular hydrocarbons.