Comparative aspects of cardiac ultrastructure, morphometry, and electrocardiography of hearts from rats fed restricted dietary copper and selenium.
Comparative aspects of cardiac ultrastructure, morphometry, and electrocardiography of hearts from rats fed restricted dietary copper and selenium.
复制标题
限制膳食铜和硒喂养的大鼠心脏超微结构、形态测定和心电图的比较。
DOI：
10.1007/bf02790067
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发表时间：
1994
影响因子：
3.9
通讯作者：
Jenkins,J
中科院分区：
文献类型：
--
作者：
Wildman,RE;Medeiros,DM;Jenkins,J
对限制饮食铜和硒后的心脏超微结构、形态测量和心电图进行了比较检查。雄性断奶 Long-Evans 大鼠被喂食铜和硒 (Cu+/Se+) 充足的饮食，或者限制铜 (Cu−) 或硒 (Se−) 的饮食，持续 8 周。第8周时，获得心电图（ECG）和dP/dts，并将心脏组织用于电子显微镜检查。经检查，Cu-大鼠贫血，心脏与体重比增大，并出现同心性肥大，其特征是左、右心室游离壁和室间隔增厚。与 Cu+/Se+ 组相比，Cu− 组中导联 aVF 的心电图记录显示 R 波振幅更大。 Se−大鼠的左心室+dP/dtmax 比 Cu+/Se+ 组和 Cu− 组更大。与 Cu+/Se+ 大鼠相比，Cu− 和 Se− 大鼠的心肌原纤维体积密度均降低。此外，Cu−大鼠表现出更大的线粒体：肌原纤维比率。铜组和硒组中均存在肌节收缩蛋白紊乱。精肌细胞还显示出水肿和线粒体碎片的证据。亚细胞改变表明与铜和硒限制相关的心脏重塑过程存在相似性。
Comparative cardiac ultrastructure, morphometry, and electrocardiography after dietary copper and selenium restriction were examined. Male weanling Long-Evans rats were fed diets that were either adequate in both copper and selenium (Cu+/Se+) or restricted in either Cu (Cu−) or Se (Se−) for 8 wk. At wk 8, electrocardiograms (ECG) anddP/dtswere obtained and heart tissue was utilized for electron microscopy. Upon examination, Cu−rats were anemic, exhibited a greater heart: body weight ratio, and developed concentric hypertrophy characterized by an enhanced thickening of the left and right ventricular free walls, and interventricular septum. ECG recordings from lead aVF in the Cu−group showed a greater R wave amplitude in comparison to the Cu+/Se+group. Se−rats recorded a greater left ventricular +dP/dtmaxthan both the Cu+/Se+and Cu−groups.Cardiac myofibril volume densities were decreased in both Cu−and Se−rats in comparison to the Cu+/Se+rats. In addition Cu−rats showed a greater mitochondria: myofibril ratio. Sarcomere contractile protein disarray was present in both the Cu−and Se−groups. Se−myocytes also showed evidence of edema and mitochondrial fragmentation. The subcellular alterations suggest that similarities exist in the cardiac remodeling processes associated with copper and selenium restrictions.