Cultured human melanocytes from black and white donors have different sunlight and ultraviolet A radiation sensitivities.
Cultured human melanocytes from black and white donors have different sunlight and ultraviolet A radiation sensitivities.
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来自黑人和白人供体的培养人类黑素细胞对阳光和紫外线 A 辐射的敏感性不同。
DOI：
10.1111/1523-1747.ep12616151
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Norris,DA
中科院分区：
文献类型：
--
作者：
Yohn,JJ;Lyons,MB;Norris,DA
短期和长期生存培养的新生儿包皮黑素细胞从黑色和白色个人进行了评估后，一次暴露于模拟阳光或紫外线A（UVA）辐射。来自黑人个体的黑素细胞比来自白色个体的黑素细胞含有显著更多的黑素（p< 0.05）。在模拟日光暴露后，黑人和白色黑素细胞具有相似的存活曲线，而在高于15 J/cm 2的UVA剂量下，黑人黑素细胞比来自白色受试者的黑素细胞对UVA细胞毒性的抗性显著更高（p < 0.05）。在模拟日光照射后，黑色或白色黑色素细胞的非程序DNA合成没有差异，并且在黑色素细胞暴露于UVA辐射后，没有可测量的非程序DNA合成。低剂量UVA（1或5 J/cm 2）对黑色和白色黑素细胞都有促有丝分裂作用。通过协方差分析，黑人和白色受试者的黑素细胞的黑素含量与UVA杀伤的敏感性显著相关（p < 0.05）;黑素含量高的黑素细胞对UVA细胞毒性具有高抵抗力，而黑素含量低的黑素细胞对UVA细胞毒性具有低抵抗力。从这些数据中，我们认为，较高的黑色素含量的黑色素细胞的黑人科目赋予增加抵抗UVA的损害。这在表皮光损伤中可能是重要的。
Short-term and long-term survival of cultured neonatal foreskin melanocytes from black and white individuals were assessed following a single exposure to simulated sunlight or ultraviolet A (UVA) radiation. Melanocytes from black individuals contained significantly more melanin than melanocytes from white individuals (p< 0.05). Black and white melanocytes had similar survival profiles following simulated sunlight exposure, whereas black melanocytes were significantly more resistant to UVA cytotoxicity than melanocytes from white subjects (p < 0.05) at UVA doses above 15 J/cm2. There was no difference in unscheduled DNA synthesis in the black or white melanocytes following simulated sunlight exposure and no unscheduled DNA synthesis was measurable following melanocyte exposure to UVA radiation. Low -dose UVA (1 or 5 J/cm2) was mitogenic to both black and white melanocytes. By analysis of co-variance, the melanin content of melanocytes of black and white subjects was significantly (p < 0.05) associated with susceptibility to UVA killing; melanocytes with high melanin content had high resistance to UVA cytotoxicity and those with low melanin content had low resistance to UVA cytotoxicity. From these data we suggest that the higher melanin content of melanocytes of black subjects confers increased resistance to UVA damage. This is likely to be of importance in epidermal photodamage.