Central injection of GalR1 agonist M617 facilitates GLUT4 expression in cardiac muscle of type 2 diabetic rats
Central injection of GalR1 agonist M617 facilitates GLUT4 expression in cardiac muscle of type 2 diabetic rats
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DOI：
10.1016/j.exger.2014.11.009
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发表时间：
2015-05-01
影响因子：
3.9
通讯作者：
Bo, Ping
中科院分区：
文献类型：
--
作者：
Fang, Penghua;Shi, Mingyi;Bo, Ping
虽然甘丙肽已被证明能增加大鼠心肌中GLUT4的表达，但关于GalR1对2型糖尿病大鼠心肌中GLUT4表达的影响尚无文献报道。本研究的目的是确定侧脑室注射GalR1激动剂M617是否会增加2型糖尿病大鼠心肌中GLUT4的表达。将实验大鼠分为4组：健康组和2型糖尿病药物组大鼠在5亩L人工脑脊液中注射10 nm/kg/dM617，连续21d，对照组给予5亩L载体注射。对血样进行血糖和胰岛素浓度分析。收集心肌组织，进行GLUT4基因表达和蛋白水平的测定。目前的研究结果显示，两个M617治疗组的空腹血糖水平都低于各自的对照组。两个M617治疗组的胰岛素水平均低于各自的对照组。此外，两种药物组大鼠心肌中GLUT4含量均高于各自的对照组。M617处理组与各对照组相比，GLUT4基因表达和GLUT4蛋白水平均升高。这些观察表明，GalR1激动剂M617通过其中心GalR1作用，可以促进2型糖尿病大鼠心肌中GLUT4的表达和增加GLUT4的含量。中心型GalR1可能在2型糖尿病大鼠心肌糖代谢稳态的调节中发挥重要作用。(C)2014 Elsevier Inc.保留所有权利。
Although galanin has been shown to increase GLUT4 expression in the cardiacmuscle of rats, there is no literature available about the effect of GalR1 on GLUT4 expression in the cardiac muscle of type 2 diabetic rats. The aim of this study was to determine whether intracerebroventricular injection of GalR1 agonist M617 would elevate GLUT4 expression in the cardiac muscle of type 2 diabetic rats. The rats tested were divided into four groups: rats from healthy and type 2 diabetic drug groups were injected with 10 nM/kg/dM617 in 5 mu l artificial cerebrospinal fluid for 21 days, while control received 5 mu l vehicle injections. The blood samples were analyzed for glucose and insulin concentration. Cardiac muscle was collected and processed for determination of GLUT4 mRNA expression and GLUT4 protein levels. The present findings showed that fasting blood glucose levels in both M617 treatment groups were lower compared with each control. The insulin levels in both M617 treatment groups were decreased compared with each control. Moreover, the GLUT4 content in the cardiac muscle in both drug groups was higher compared with each control. M617 treatment increased GLUT4 mRNA expression and GLUT4 protein levels compared with each control group. These observations suggest that GalR1 agonist M617, acting through its central GalR1, can promote GLUT4 expression and enhance GLUT4 content in the cardiac muscle of type 2 diabetic rats. Central GalR1 may play a significant role in regulation of glucosemetabolic homeostasis in the cardiac muscle of type 2 diabetic rats. (C) 2014 Elsevier Inc. All rights reserved.