Single cells as biosensors for chemical separations.
Single cells as biosensors for chemical separations.
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单细胞作为化学分离的生物传感器。
DOI：
10.1126/science.7809609
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Scheller,RH
中科院分区：
文献类型：
--
作者：
Shear,JB;Fishman,HA;Allbritton,NL;Garigan,D;Zare,RN;Scheller,RH
A biosensor system based on the response of living cells was demonstrated that can detect specific components of a complex mixture fractionated by a microcolumn separation technique. This system uses ligand-receptor binding and signal-transduction pathways to biochemically amplify the presence of an analyte after electrophoretic separation. The transduced signal was measured by means of two approaches: (i) fluorescence determination of intracellular calcium concentrations in one or more rat PC-12 cells and (ii) measurement of transmembrane current in aXenopus laevisoocyte microinjected with messenger RNA that encodes a specific receptor. This analysis system has the potential to identify biologically active ligands present in a complex mixture with exceptional sensitivity and selectivity.
