Development of an Au/ZnO thin film surface plasmon resonance-based biosensor immunoassay for the detection of carbohydrate antigen 15-3 in human saliva
Development of an Au/ZnO thin film surface plasmon resonance-based biosensor immunoassay for the detection of carbohydrate antigen 15-3 in human saliva
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DOI：
10.1016/j.clinbiochem.2012.09.001
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发表时间：
2012-12-01
影响因子：
2.8
通讯作者：
Lin, Chii-Wann
中科院分区：
文献类型：
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作者：
Liang, Yang-Hung;Chang, Chia-Chen;Lin, Chii-Wann
目的：为了评价一种新的表面等离子体共振（SPR）系统用于直接测量人唾液中的肿瘤标志物碳水化合物抗原15-3（CA 15 -3），设计和方法：我们使用两种不同的表面等离子体共振（SPR）系统测量人唾液中肿瘤标志物碳水化合物抗原15-3（CA 15 -3）的存在。为了比较基于薄膜Au/ZnO的SPR生物传感器和Biacore SPR系统的灵敏度，我们在唾液中制备了CA 15 -3样品，并在不同浓度的CA 15 - 3下分析了样品的强度响应。以Au/ZnO薄膜为基础的SIR系统对人唾液中CA 15 -3的线性检测范围为2.5 ~ 20 U/mL（癌症患者的截止点约为4 U/mL）。结论：薄膜Au/ZnO表面等离子体共振系统具有较高的灵敏度，可用于检测唾液中CA 15 -3的含量，且不需要样品浓缩。(C)2012年加拿大临床化学家协会。爱思唯尔公司出版All rights reserved.
Objective: To evaluate a novel surface plasmon resonance (SPR) system for the direct measurement of tumor marker carbohydrate antigen 15-3 (CA15-3) in human saliva.Design and methods: We measured the presence of the tumor marker carbohydrate antigen 15-3 (CA15-3) in human saliva using 2 different surface plasmon resonance (SPR) systems. To compare the sensitivity of an SPR biosensor based on thin-film Au/ZnO and the Biacore SPR system, we prepared CA15-3 samples in saliva and analyzed intensity responses to the samples at various concentrations of CA15-3.Results: The linear detection range of CA15-3 in human saliva with the SIR system based on thin-film Au/ZnO was 2.5-20 U/mL (the cut-off point in cancer patients is around 4 U/mL). The linear range with the Biacore SPR system was 40-300 U/mLConclusions: These results show that thin-film Au/ZnO-based SPR systems have higher sensitivity and can be used for measuring the levels of CA15-3 in human saliva without concentrating the samples. (C) 2012 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.