Requirement of Mis6 centromere connector for localizing a CENP-A-like protein in fission yeast
Requirement of Mis6 centromere connector for localizing a CENP-A-like protein in fission yeast
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DOI：
10.1126/science.288.5474.2215
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发表时间：
2000-06-23
期刊：
影响因子：
56.9
通讯作者：
Yanagida, M
中科院分区：
文献类型：
--
作者：
Takahashi, K;Chen, ES;Yanagida, M
哺乳动物着丝粒含有着丝粒特异性组蛋白 H3 变体 CENP-A，其掺入有限的染色体区域可能对有丝分裂期间的着丝粒功能和染色体分离很重要。然而，CENP-A定位的调控及其作用尚不清楚。在这里，我们报告裂殖酵母同源物 SpCENP-A 对于建立与平等染色体分离相关的着丝粒染色质至关重要。 SpCENP-A 与非重复内部着丝粒的结合依赖于 Mis6，Mis6 是一种在细胞周期 G(1)-S 期发挥作用的重要着丝粒连接蛋白。 Mis6 可能是招募 SpCENP-A 以形成姐妹着丝粒的正确连接所必需的。
Mammalian kinetochores contain the centromere-specific histone H3 Variant CENP-A, whose incorporation into limited chromosomal regions may be important for centromere function and chromosome segregation during mitosis. However, regulation of CENP-A localization and its role have not been clear. Here we report that the fission yeast homolog SpCENP-A is essential for establishing centromere chromatin associated with equal chromosome segregation. SpCENP-A binding to the nonrepetitious inner centromeres depended on Mis6, an essential centromere connector protein acting during G(1)-S phase of the cell cycle. Mis6 is likely required for recruiting SpCENP-A to form proper connection of sister centromeres.