Alveolar epithelial injury and pleural empyema in acute P. aeruginosa pneumonia in anesthetized rabbits.
Alveolar epithelial injury and pleural empyema in acute P. aeruginosa pneumonia in anesthetized rabbits.
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麻醉兔急性铜绿假单胞菌肺炎的肺泡上皮损伤和胸膜脓胸。
DOI：
10.1152/jappl.1993.75.4.1661
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Matthay,MA
中科院分区：
文献类型：
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作者：
Wiener-Kronish,JP;Sakuma,T;Kudoh,I;Pittet,JF;Frank,D;Dobbs,L;Vasil,ML;Matthay,MA
我们在麻醉通气兔中建立了急性铜绿假单胞菌肺炎的实验模型，以确定是否会发生细菌诱导的肺泡上皮损伤以及损伤对胸膜腔的影响。剂量反应研究确定，需要10（9）个铜绿假单胞菌（野生型菌株，PAO-1）菌落形成单位才能损伤上皮屏障并导致胸腔积脓伴渗出性胸腔积液（包含滴注的肺泡蛋白示踪剂和铜绿假单胞菌）。通过比较3株铜绿假单胞菌的几种胞外毒力产物，探讨铜绿假单胞菌致肺和胸膜损伤的机制。PAO-S21在一个调节基因中携带一个插入突变，阻止外切酶S的产生，没有导致肺或胸膜损伤。PAO-R1携带控制弹性蛋白酶和碱性蛋白酶产生的调节基因的缺失，引起与PAO-1相同程度的肺和胸膜损伤。灌注PLC-SRN，其中有两个结构基因编码磷脂酶C活性删除，导致肺泡上皮损伤的中度减少。虽然可能涉及其他产物，但外切酶S和磷脂酶C在介导兔急性铜绿假单胞菌肺炎中肺泡上皮屏障损伤中是重要的。
We developed an experimental model of acute Pseudomonas aeruginosa pneumonia in anesthetized ventilated rabbits to determine whether bacterial-induced injury to the alveolar epithelium would occur and the effect of the injury on the pleural space. Dose-response studies established that 10(9) colony-forming units of P. aeruginosa (wild-type strain, PAO-1) were required to injure the epithelial barrier and to cause pleural empyema with exudative pleural effusions that contained both the instilled alveolar protein tracer and P. aeruginosa. We explored the mechanisms of P. aeruginosa-induced lung and pleural injury by using three isogenic bacterial strains to compare several extracellular virulence products. PAO-S21, which carries an insertion mutation in a regulatory gene that prevents the production of exoenzyme S, resulted in no lung or pleural injury. PAO-R1, which carries a deletion in a regulatory gene that controls the production of elastase and alkaline protease, caused the same degree of lung and pleural injury as PAO-1 did. Instillation of PLC-SRN, which has both structural genes encoding phospholipase C activity deleted, resulted in a moderate reduction in alveolar epithelial injury. Although other products may be involved, exoenzyme S and phospholipase C are important in mediating injury to the alveolar epithelial barrier in acute P. aeruginosa pneumonia in rabbits.