Randomly amplified polymorphic DNA technique in molecular identification of germplasms of Carthamus tinctorius L.
Randomly amplified polymorphic DNA technique in molecular identification of germplasms of Carthamus tinctorius L.
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发表时间：
2003
影响因子：
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通讯作者：
G. Mei
中科院分区：
文献类型：
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作者：
G. Mei
目的：从分子水平研究红花种内变异，为红花种质间的分子鉴定提供依据。方法：采用随机扩增多态性DNA （RAPD）技术和统计学方法，对中国9个省22个红花品种的遗传多样性和亲缘关系进行分析。结果：根据RAPD特征图和聚类分析将22个品种划分为7个类群。结论：红花存在种内变异。研究结果为红花品种间的鉴定和亲缘关系研究提供了依据。
Objective:To study the intraspecific variance of safflower at molecular level, providing the basis for molecular identification among germplasms of Carthamus tinctorius L. . Methods: The genetic diversities and the relationship among 22 cultivars of safflower (Carthamus tinctorius L. ) from 9 provinces of China were analyzed by randomly amplified polymorphic DNA (RAPD) technique and statistical methods. Results: The 22 cultivars were divided into 7 groups based on the characteristic maps of RAPD and cluster analysis. Conclusion: There are intraspecific variances in safflower. The results provide a basis for identification and genetic relationship study among cultivars of Carthamus tinctorius L. .