Peripodial cells regulate proliferation and patterning of Drosophila imaginal discs
Peripodial cells regulate proliferation and patterning of Drosophila imaginal discs
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DOI：
10.1016/s0092-8674(00)00125-2
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发表时间：
2000-10-13
期刊：
影响因子：
64.5
通讯作者：
Schubiger, G
中科院分区：
文献类型：
--
作者：
Gibson, MC;Schubiger, G
细胞在发育过程中采用多种信号机制来建立空间模式。没有什么地方比果蝇更能理解这一点了，果蝇的四肢和眼睛是由称为成虫盘的离散上皮囊产生的。模式形成的分子遗传学分析通常将椎间盘视为单个上皮片。然而，从解剖学上讲，椎间盘包含被称为足周膜的鳞状上皮覆盖的柱状细胞单层。在这里，我们证明在发育过程中，足周细胞通过基于微管的顶端延伸向椎间盘柱状细胞发出信号。消融和靶向基因错误表达实验表明，足周细胞信号传导有助于眼睛和翅膀原基的生长控制和模式形成。这些发现挑战了椎间盘为单层的传统观点，并为果蝇附肢发育中的足周细胞功能提供了基础证据。
Cells employ a diverse array of signaling mechanisms to establish spatial patterns during development. Nowhere is this better understood than in Drosophila, where the limbs and eyes arise from discrete epithelial sacs called imaginal discs. Molecular-genetic analyses of pattern formation have generally treated discs as single epithelial sheets. Anatomically, however, discs comprise a columnar cell monolayer covered by a squamous epithelium known as the peripodial membrane. Here we demonstrate that during development, peripodial cells signal to disc columnar cells via microtubule-based apical extensions. Ablation and targeted gene misexpression experiments demonstrate that peripodial cell signaling contributes to growth control and pattern formation in the eye and wing primordia. These findings challenge the traditional view of discs as monolayers and provide foundational evidence for peripodial cell function in Drosophila appendage development.