Cloning and expression analysis of some genes involved in the phosphoinositide and phospholipid signaling pathways from maize (Zea mays L.).
Cloning and expression analysis of some genes involved in the phosphoinositide and phospholipid signaling pathways from maize (Zea mays L.).
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玉米（Zea mays L.）参与磷酸肌醇和磷脂信号通路的一些基因的克隆和表达分析。
DOI：
10.1016/j.gene.2008.09.004
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发表时间：
2008
期刊：
影响因子：
3.5
通讯作者：
Juren Zhang
中科院分区：
文献类型：
--
作者：
Zhenhua Sui;Linyuan Niu;Guidong Yue;A. Yang;Juren Zhang
研究表明，磷脂酰肌醇和磷脂信号通路在植物生长发育和对环境胁迫的响应中起着重要作用。然而，很少有人知道磷酸肌醇和磷脂信号通路在玉米（玉米）。为了更好地了解玉米磷脂酰肌醇和磷脂信号通路相关基因的功能，本研究采用RACE（rapid amplification of cDNA ends）或电子克隆结合PCR的方法获得了玉米磷脂酰肌醇和磷脂信号通路相关基因ZmPIS 2、ZmPLC 2、ZmDGK 1、ZmDGK 2和ZmDGK 3的cDNA序列。对根、茎、叶、雄穗和穗5个组织的cDNA进行RT-PCR分析，结果表明，这5个cDNA以及ZmPIS、ZmPLC、ZmPLD在不同组织中的表达模式存在差异。为了确定不同的环境条件，如寒冷，干旱和各种植物激素（脱落酸，吲哚-3-乙酸和赤霉酸）对基因表达的影响，我们分析了实时（RT-PCR）的表达，并发现这些基因家族的不同亚型参与磷脂和肌醇信号通路具有特定的表达模式。这些基因可能参与了植物对环境胁迫的反应，但功能不同。对磷脂酰肌醇和磷脂信号通路相关基因的分离和表达模式的分析为进一步研究玉米磷脂酰肌醇和磷脂信号通路提供了良好的基础，也是对我们理解植物磷脂酰肌醇和磷脂信号通路的一个新的补充。
Previous studies have indicated the phosphoinositide and phospholipid signaling pathways play a key role in plant growth, development and responses to environmental stresses. However, little is known about the phosphoinositide and phospholipid signaling pathways in maize (Zea mays L.). To better understand the function of genes involved in the phosphoinositide and phospholipid signaling pathways in maize, the cDNA sequences of ZmPIS2, ZmPLC2, ZmDGK1, ZmDGK2 and ZmDGK3 were obtained by RACE (rapid amplification of cDNA ends) or in silico cloning combined with PCR. RT-PCR analysis of cDNA from five tissues (roots, stems, leaves, tassels, and ears) indicated that the expression patterns of the five cDNAs we isolated as well as ZmPIS, ZmPLC, ZmPLD varied in different tissues. To determine the effects of different environmental conditions such as cold, drought and various phytohormones (abscisic acid, indole-3-acetic acid and gibberellic acid) on gene expression, we analyzed expression by Real-Time (RT-PCR), and found that the different isoforms of these gene families involved in the phosphoinositide and phospholipid signaling pathways have specific expression patterns. Our results suggested that these genes may be involved in the responses to environmental stresses, but have different functions. The isolation and analysis of expression patterns of genes involved in the phosphoinositide and phospholipid signaling pathways provides a good basis for further research of the phosphoinositide and phospholipid signaling pathways in maize and is a novel supplement to our comprehension of these pathways in plants.
影响因子：
11.6
作者：
Stenzel, Irene;Ischebeck, Till;Heilmann, Ingo
通讯作者：
Heilmann, Ingo