miR-449b inhibits the proliferation of SW1116 colon cancer stem cells through downregulation of CCND1 and E2F3 expression
miR-449b inhibits the proliferation of SW1116 colon cancer stem cells through downregulation of CCND1 and E2F3 expression
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DOI：
10.3892/or.2013.2465
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发表时间：
2013-07-01
期刊：
影响因子：
4.2
通讯作者：
Chen, Zongyou
中科院分区：
文献类型：
--
作者：
Fang, Yantian;Gu, Xiaodong;Chen, Zongyou
结直肠癌是全球癌症相关死亡率的主要原因之一。肿瘤干细胞是存在于肿瘤组织中的具有干细胞性质的细胞群，是肿瘤形成和转移的根源。CCND 1和E2 F3在细胞周期调控中起重要作用。CCND 1和E2 F3的3 'UTR含有miR-449结合位点。通过阻断pre-miR-449 b和抑制miR-449 b，我们发现结肠癌干细胞的细胞周期、细胞增殖能力和细胞周期调控蛋白表达水平发生了改变。CCND 1、E2 F3和miR-449 b之间的相关性表明，miR-449 b可下调CCND 1和E2 F3的表达。这反过来又降低了结肠癌干细胞的增殖能力。这些数据表明，miR-449 b在结肠癌干细胞中发挥肿瘤抑制作用。
Colorectal cancer is one of the leading causes of cancer-related mortality worldwide. Cancer stem cells are cell populations with stem cell nature presenting in tumor tissues and are the root of tumor formation and metastasis. CCND1 and E2F3 play important roles in cell cycle regulation. The 3'UTRs of CCND1 and E2F3 contain miR-449 binding sites. By transfecting pre-miR-449b and inhibiting miR-449b, we found that cell cycle, cell proliferation ability and cell cycle regulatory protein expression levels of colon cancer stem cells were altered. The correlation between CCND1, E2F3 and miR-449b showed that miR-449b could downregulate CCND1 and E2F3 expression. This, in turn, reduced the proliferative ability of colon cancer stem cells. These data suggest that miR-449b plays a tumor-suppressive role in colon cancer stem cells.