Galangin inhibits proliferation of HepG2 cells by activating AMPK via increasing the AMP/TAN ratio in a LKB1-independent manner
Galangin inhibits proliferation of HepG2 cells by activating AMPK via increasing the AMP/TAN ratio in a LKB1-independent manner
复制标题
高良姜素通过以不依赖 LKB1 的方式增加 AMP/TAN 比率来激活 AMPK，从而抑制 HepG2 细胞的增殖
DOI：
10.1016/j.ejphar.2013.08.026
复制
发表时间：
2013-10-15
影响因子：
5
通讯作者：
Luo, Hui
中科院分区：
文献类型：
--
作者：
Zhang, Haitao;Li, Ning;Luo, Hui
高良姜素是从高良姜中提取的一种黄酮醇，在我国南方被用作食品添加剂，可诱导HepG 2细胞凋亡和自噬，从而抑制HepG 2细胞的增殖。在这项研究中，我们证明高良姜通过增加HepG 2细胞中AMP/TAN的比例来诱导自噬。它刺激磷酸腺苷活化蛋白激酶（AMPK）和LKB 1的磷酸化，但抑制AKT和mTOR的磷酸化。AMPK激活的抑制抑制了mTOR的去磷酸化，从而阻断了高良姜素诱导的自噬。AMPIC激活高良姜素似乎是独立的LKB 1信号通路，因为LKB 1的siRNA下调未能影响高良姜素诱导的自噬。总的来说，这些发现证明了高良姜素如何以LKB 1非依赖性方式通过激活AIVIPK诱导自噬的新机制。因此，自噬的诱导可以反映高良姜素在肝癌细胞中的抗增殖作用。(C)© 2013 Elsevier B. V.版权所有
Galangin, a flavonol derived from Alpinia officinarurn Hance and used as food additives in southern China, induces apoptosis and autophagy to suppress the proliferation of HepG2 cells. In this study, we demonstrated that galangin induced autophagy by increasing the ratio of AMP/TAN in HepG2 cells. It stimulated the phosphorylation of adenosine monophosphate-activated protein kinase (AMPK) and LKB1, but inhibited the phosphorylation of AKT and mTOR. Inhibition of AMPK activation suppressed the dephosphorylation of mTOR to block galangin-induced autophagy. AMPIC activation by galangin appeared to be independent of the LKB1 signaling pathway because the down-regulation of LKB1 by its siRNA failed to affect galangin-induced autophagy. Collectively, the findings demonstrated a novel mechanism of how galangin induces autophagy via activating AIVIPK in a LKB1-independent manner. The induction of autophagy can thus reflect the anti-proliferation effect of galangin in HCC cells. (C) 2013 Elsevier B.V. All rights reserved