ESTIMATION OF THE NUMERICAL DENSITY OF SYNAPSES IN RAT NEOCORTEX - COMPARISON OF THE DISECTOR WITH AN UNFOLDING METHOD
ESTIMATION OF THE NUMERICAL DENSITY OF SYNAPSES IN RAT NEOCORTEX - COMPARISON OF THE DISECTOR WITH AN UNFOLDING METHOD
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DOI：
10.1016/0165-0270(88)90003-9
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发表时间：
1988-04-01
影响因子：
3
通讯作者：
JONES, DG
中科院分区：
文献类型：
--
作者：
CALVERLEY, RKS;BEDI, KS;JONES, DG
本文用展开法和分割法两种体视学方法对28日龄正常大鼠顶叶皮质分子层突触的数密度进行了估计。对于展开技术，假设突触形成多分散的盘状颗粒群。该技术还依赖于关于突触在截面平面中的大小、形状和投影的各种假设，这些假设产生的结果在未知程度上有偏差。这种简化的假设对于剖分法是不必要的。平均值。+-。S.E.M.发现突触的数密度为2.16 ± 0.001。0.14. times. 109/mm3。这与2.41 ± 0.05的值没有显著差异（配对t检验; P < 0.05）。0.22. times. 109/mm 3，使用展开技术获得，并进行截断校正（“帽丢失”）。当使用展开方法而不对丢失的帽进行校正时，数密度为1.89 ± 1。10.0. times. 109/mm 3，该值与使用剥离器方法获得的值也没有显著差异。用两种方法测定突触颗粒的平均投影高度（.hivin.Hs），结果无显著差异。当考虑到最终估计值的方差和执行所述程序所需的时间时，估计disector方法比具有封端校正的展开方法效率高约86%。尽管展开方法可以给出与分割器方法基本相似的突触数密度值，但它有偏差，效率低得多。它的结论是，因此，分割器的方法是上级的方法，用于估计的数量和大小的颗粒，如突触，特别是当它们具有复杂的空间配置。
The numerical density of synapses in the molecular layer of the parietal cortex of normal 28-day-old rats has been estimated by using two stereological methods, namely an unfolding technique and the disector method. For the unfolding technique it was assumed that the synapses formed a polydispersed population of disc-shaped particles. The technique also relies upon various assumptions regarding the size, shape and projection of synapses in the plane of section, these assumptions producing a result that is biased to an unknown extent. Such simplifying assumptions are unnecessary for the disector method. The mean .+-. S.E.M. numerical density of synapses was found to be 2.16 .+-. 0.14 .times. 109/mm3 using the disector method. This was not significantly different (paired t-test; P < 0.05) from the value of 2.41 .+-. 0.22 .times. 109/mm3 obtained using the unfolding technique with correction for truncation (''lost caps''). When the unfolding method was used without a correction for lost caps the numerical density was 1.89 .+-. 10.0 .times. 109/mm3, a value also not significantly different from that obtained using the disector method. The mean projected height (.hivin.Hs) ofthe synaptic particles was also determined using both procedures; the results were not significantly different. When the variances of the final estimates and the time taken to carry out the procedures described were taken into account, it was estimated that the disector method was about 86% more efficient than the unfolding method with capping correction. Although the unfolding method can give essentially similar values for the numerical density of synapses to those obtained by the disector method, it is biased and much less efficient. It is concluded, therefore, that the disector method is the superior method for the estimation of the number and size of particles such as synapses, particularly when they have complex spatial configurations.