Highly efficient 3D fluorescence microscopy with a scanning laser optical tomograph
Highly efficient 3D fluorescence microscopy with a scanning laser optical tomograph
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DOI：
10.1364/oe.19.005419
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发表时间：
2011-03-14
期刊：
影响因子：
3.8
通讯作者：
Heisterkamp, Alexander
中科院分区：
文献类型：
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作者：
Lorbeer, Raoul-Amadeus;Heidrich, Marko;Heisterkamp, Alexander
光学投影层析成像（OPT）被证明是有用的三维跟踪的荧光信号在生物模型生物体的大小高达几毫米。这种层析成像技术检测吸收以及荧光，以创建多模态三维数据。虽然样品的吸收被非常快地检测到，但通常收集不到0.1%的荧光光子。低效率可导致辐射剂量依赖性伪影，例如光漂白和光毒性。为了最大限度地减少这些影响以及由于使用CCD或CMOS相机芯片而引入的伪影，我们构建了扫描激光光学断层扫描仪（SLOT）。与传统的荧光OPT相比，我们的第一个SLOT将光子收集效率提高了一百倍。(C)2011年美国光学学会
Optical Projection Tomography (OPT) proved to be useful for the three-dimensional tracking of fluorescence signals in biological model organisms with sizes up to several millimeters. This tomographic technique detects absorption as well as fluorescence to create multimodal three-dimensional data. While the absorption of a specimen is detected very fast usually less than 0.1% of the fluorescence photons are collected. The low efficiency can result in radiation dose dependent artifacts such as photobleaching and phototoxicity. To minimize these effects as well as artifacts introduced due to the use of a CCD- or CMOS-camera-chip, we constructed a Scanning Laser Optical Tomograph (SLOT). Compared to conventional fluorescence OPT our first SLOT enhanced the photon collection efficiency a hundredfold. (C) 2011 Optical Society of America