The rice thylakoid membrane-bound ascorbate peroxidase OsAPX8 functions in tolerance to bacterial blight.
The rice thylakoid membrane-bound ascorbate peroxidase OsAPX8 functions in tolerance to bacterial blight.
复制标题
水稻类囊体膜结合抗坏血酸过氧化物酶 OsAPX8 具有抗白叶枯病的功能
DOI：
10.1038/srep26104
复制
发表时间：
2016-05-17
影响因子：
4.6
通讯作者：
Zhai W
中科院分区：
文献类型：
--
作者：
Jiang G;Yin D;Zhao J;Chen H;Guo L;Zhu L;Zhai W
类囊体膜结合抗坏血酸过氧化物酶（tAPX）是一种主要的H2 O2清除酶。为了阐明其在水稻白叶枯病抗性中的功能，我们制备了过量表达和抑制tAPX（OsAPX 8）的水稻品系。过表达系表现出对细菌病原体的耐受性增强。RNA干扰（RNAi）株系比对照植物敏感得多。对这些转基因植株中H2 O2含量的进一步分析表明，在细菌病原体的攻击下，OsAPX 8过表达植株的H2 O2积累显著低于野生型和RNAi植株。有趣的是，H2 O2是导致无主效抗性基因水稻叶片严重脱水和枯萎的最重要因素，而不是引起敏感性的原因。此外，根据H2 O2的水平，可能会发生壁收紧或松动。此外，OsAPX 8与感病蛋白Os 8 N3/Xa 13相互作用，抑制了OsAPX 8对白叶枯病的抗性反应。
Thylakoid membrane-bound ascorbate peroxidase (tAPX) is a major H2O2-scavenging enzyme. To clarify its functions in tolerance to rice bacterial blight, we produced rice lines overexpressing and suppressingtAPX(OsAPX8). The overexpressing lines exhibited increased tolerance to bacterial pathogen. The RNA interference (RNAi) lines were considerably more sensitive than the control plant. Further analysis of the H2O2content in these transgenic plants indicated that the H2O2accumulation ofOsAPX8-overexpressing plants was considerably less than that of wild-type and RNAi plants upon challenge with bacterial pathogen. Interestingly, H2O2was the most important factor for the serious leaf dehydration and withering of rice without major resistance genes and was not the cause of hypersensitivity. It addition, wall tightening or loosening can occur according to the level of H2O2. In addition, OsAPX8 interacted with the susceptibility protein Os8N3/Xa13, and their binding repressed the reaction of OsAPX8 in tolerance to bacterial blight.