The Eucalyptus Tonoplast Intrinsic Protein (TIP) Gene Subfamily: Genomic Organization, Structural Features, and Expression Profiles.
The Eucalyptus Tonoplast Intrinsic Protein (TIP) Gene Subfamily: Genomic Organization, Structural Features, and Expression Profiles.
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DOI：
10.3389/fpls.2016.01810
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发表时间：
2016
影响因子：
5.6
通讯作者：
Maia IG
中科院分区：
文献类型：
--
作者：
Rodrigues MI;Takeda AA;Bravo JP;Maia IG
植物水通道蛋白参与多种生理过程，包括生长发育和对胁迫的适应。本研究对经济价值较高的桉树液泡膜固有蛋白(TIP)基因亚家族进行了研究。对巨桉基因组的全基因组调查显示，巨桉基因组中存在11个假定的TIP基因(称为EgTIP)，它们分别被系统发育归入每一个经典的TIP1-5组。同源建模证实了在所鉴定的EgTIP中存在两个高度保守的NPA(ASN-Pro-Ala)基序。观察到相应选择性过滤器中的残基变化，这可能反映了EgTIP底物专一性的差异。除EgTIP5.1外，所有EgTIP基因均被转录，且大部分基因呈现器官/组织富集性表达。对EgTIP启动子的检测表明，存在与非生物胁迫和激素反应有关的常见顺式调控元件，表明已鉴定的基因参与了非生物胁迫反应。与这些观察结果一致的是，额外的基因表达谱显示在聚乙二醇施加的渗透胁迫下表达增加。总之，研究结果表明，这些新的EgTIP可能在功能上与桉树对逆境的适应有关。
Plant aquaporins are water channels implicated in various physiological processes, including growth, development and adaptation to stress. In this study, the Tonoplast Intrinsic Protein (TIP) gene subfamily of Eucalyptus, an economically important woody species, was investigated and characterized. A genome-wide survey of the Eucalyptus grandis genome revealed the presence of eleven putative TIP genes (referred as EgTIP), which were individually assigned by phylogeny to each of the classical TIP1–5 groups. Homology modeling confirmed the presence of the two highly conserved NPA (Asn-Pro-Ala) motifs in the identified EgTIPs. Residue variations in the corresponding selectivity filters, that might reflect differences in EgTIP substrate specificity, were observed. All EgTIP genes, except EgTIP5.1, were transcribed and the majority of them showed organ/tissue-enriched expression. Inspection of the EgTIP promoters revealed the presence of common cis-regulatory elements implicated in abiotic stress and hormone responses pointing to an involvement of the identified genes in abiotic stress responses. In line with these observations, additional gene expression profiling demonstrated increased expression under polyethylene glycol-imposed osmotic stress. Overall, the results obtained suggest that these novel EgTIPs might be functionally implicated in eucalyptus adaptation to stress.
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影响因子：
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