Association of resistin with impaired membrane fluidity of red blood cells in hypertensive and normotensive subjects: an electron paramagnetic resonance study.
Association of resistin with impaired membrane fluidity of red blood cells in hypertensive and normotensive subjects: an electron paramagnetic resonance study.
复制标题
高血压和正常血压受试者抵抗素与红细胞膜流动性受损的关联：电子顺磁共振研究。
DOI：
10.1007/s00380-015-0755-0
复制
发表时间：
2016
影响因子：
1.5
通讯作者：
Kazushi Tsuda
中科院分区：
文献类型：
--
作者：
長谷川由美;難波秀行;櫻井洋一;王堂 哲;Kazushi Tsuda
细胞膜物理性质的异常可能与高血压密切相关。最近的证据表明，抵抗素可能积极参与胰岛素抵抗、糖尿病、高血压和其他循环系统疾病的病理生理学。本研究旨在探讨高血压中血浆抵抗素、氧化应激和膜流动性（膜微粘度的倒数）之间的可能关系。我们使用电子顺磁共振 (EPR) 和自旋标记方法测量了高血压和血压正常男性的红细胞 (RBC) 膜流动性。高血压男性红细胞 (RBC) 膜 EPR 谱中自旋标记试剂（5-硝基氧硬脂酸酯）的有序参数 (S) 显着高于血压正常男性，表明高血压患者膜流动性降低。血浆抵抗素水平与收缩压和 8-异前列腺素 F2α 水平（氧化应激指数）相关。此外，红细胞的有序参数（S）与血浆抵抗素和血浆8-isoPG F2α显着相关，表明红细胞膜流动性降低可能与高抵抗素血症和氧化应激增加有关。多元回归分析表明，调整混杂因素后，血浆抵抗素可能是红细胞膜流动性的独立决定因素。 EPR 研究表明，抵抗素可能与红细胞流变行为受损和高血压的微循环功能障碍密切相关，至少部分是通过氧化应激依赖性机制。
Abnormalities in physical properties of the cell membranes may strongly be linked to hypertension. Recent evidence indicates that resistin may actively participate in the pathophysiology of insulin resistance, diabetes mellitus, hypertension and other circulatory disorders. The present study was undertaken to investigate the possible relationships among plasma resistin, oxidative stress and membrane fluidity (a reciprocal value of membrane microviscosity) in hypertension. We measured the membrane fluidity of red blood cells (RBCs) in hypertensive and normotensive men using an electron paramagnetic resonance (EPR) and spin-labeling method. The order parameter (S) for the spin-label agents (5-nitroxide stearate) in EPR spectra of red blood cell (RBC) membranes was significantly higher in hypertensive men than in normotensive men, indicating that membrane fluidity was decreased in hypertension. Plasma resistin levels were correlated with systolic blood pressure and 8-iso-prostaglandin F2α levels (an index of oxidative stress). Furthermore, the order parameter (S) of RBCs significantly correlated with plasma resistin and plasma 8-isoPG F2α, suggesting that reduced membrane fluidity of RBCs might be associated with hyperresistinemia and increased oxidative stress. Multivariate regression analysis showed that, after adjustment for confounding factors, plasma resistin might be an independent determinant of membrane fluidity of RBCs. The EPR study suggests that resistin might have a close correlation with impaired rheologic behavior of RBCs and microcirculatory dysfunction in hypertension, at least in part, via an oxidative stress-dependent mechanism.