Expression of interleukin 10 in human melanoma.
Expression of interleukin 10 in human melanoma.
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DOI：
10.1038/bjc.1994.469
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发表时间：
1994-12
影响因子：
8.8
通讯作者：
Diamantstein, T
中科院分区：
文献类型：
--
作者：
Kruger-Krasagakes, S;Krasagakis, K;Garbe, C;Schmitt, E;Huls, C;Blankenstein, T;Diamantstein, T
应用逆转录聚合酶链反应（RT-PCR）技术检测人恶性黑色素瘤组织中白细胞介素10（IL-10）mRNA的表达。IL-10 mRNA在原发性黑色素瘤和转移性黑色素瘤组织中的选择性表达与正常皮肤相比。此外，在13个黑素瘤细胞系中的3个中检测到IL-10 mRNA和生物活性IL-10的强表达。正常黑素细胞始终表达低水平的IL-10 mRNA，但不产生可检测的IL-10蛋白，也没有角质形成细胞或成纤维细胞。黑色素瘤细胞系产生生物活性IL-10表明黑色素瘤病变中的IL-10 mRNA可能至少部分来自肿瘤细胞本身。然而，肿瘤浸润细胞也可能是黑色素瘤组织中IL-10的来源。黑色素瘤病变中IL-10的存在可能有助于假定的抗黑色素瘤免疫应答的“麻痹”。
The expression of interleukin 10 (IL-10) mRNA in human malignant melanoma was investigated by reverse transcriptase polymerase chain reaction analysis. Selective expression of IL-10 mRNA in tissues of primary melanomas and melanoma metastases was found in comparison with normal skin. In addition, strong expression of IL-10 mRNA and of biologically active IL-10 was detected in 3 out of 13 melanoma cell lines. Normal melanocytes consistently expressed low levels of IL-10 mRNA but did not produce detectable IL-10 protein, nor did keratinocytes or fibroblasts. The production of biologically active IL-10 by melanoma cell lines suggests that IL-10 mRNA in melanoma lesions may derive at least in part from the tumour cells themselves. Tumour-infiltrating cells, however, could also be a source of IL-10 in melanoma tissues. The presence of IL-10 in melanoma lesions may contribute to the postulated 'paralysis' of an anti-melanoma immune response.