A microsatellite genetic linkage map of human chromosome 18.
A microsatellite genetic linkage map of human chromosome 18.
复制标题
人类18号染色体的微卫星遗传连锁图。
DOI：
10.1006/geno.1993.1008
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发表时间：
1993
期刊：
影响因子：
4.4
通讯作者：
Gilliam,TC
中科院分区：
文献类型：
--
作者：
Straub,RE;Speer,MC;Luo,Y;Rojas,K;Overhauser,J;Ott,J;Gilliam,TC
We isolated nine new microsatellite markers from chromosome 18 and further characterized and mapped eight microsatellites developed in other laboratories. We have constructed a framework linkage map of chromosome 18 that includes 14 microsatelllte markers (12 dinucleotide and 2 tetranucleotide) and 2 RFLP markers. Cytogenetic localization for the microsatellites was performed by PCR amplification of 18 somatic cell hybrids containing different deletions of chromosome 18. Twelve of the microsatellites and one of the RFLPs have heterozygosities greater than 70%. The average heterozygosity of the markers included in the map is 72%. In addition, we have made provisional placements of 3 more microsatellite markers and 2 more RFLP markers. The map lengths (in Kosambi centimorgans) are as follows: sex-averaged, 109.3 cM; male, 72.4 cM; female, 161.2 cM. The average distance between markers in the sex-averaged map is 7.3 cM, and the largest gap between markers is 16.7 cM. Analysis of the data for differences in the female:male map distance ratio revealed significant evidence for a constant difference in the ratio (χ2= 32.25;df= 1;P< 0.001; ratio = 2.5:1). Furthermore, there was significant evidence in favor of a variable female:male map distance ratio across the chromosome compared to a constant distance ratio (χ2= 27.78;df= 14;P= 0.015). To facilitate their use in genomic screening for disease genes, all of the microsatelllte markers used here can be amplified under standard PCR conditions, and most can be used in duplex PCR reactions.
