A novel antioxidant peptide, purified from Bacillus amyloliquefaciens, showed strong antioxidant potential via Nrf-2 mediated heme oxygenase-1 expression
A novel antioxidant peptide, purified from Bacillus amyloliquefaciens, showed strong antioxidant potential via Nrf-2 mediated heme oxygenase-1 expression
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DOI：
10.1016/j.foodchem.2017.06.106
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发表时间：
2018-01-12
期刊：
影响因子：
8.8
通讯作者：
Yoo, Jin Cheol
中科院分区：
文献类型：
--
作者：
Rahman, Md. Saifur;Choi, Yun Hee;Yoo, Jin Cheol
从淀粉解性芽孢杆菌CBSYD1菌株中分离纯化出一种新的抗氧化肽YD1(约1.0 kDa)，该抗氧化肽对革兰氏阳性菌、革兰氏阴性菌和多药耐药菌(MDR)均有一定的抗氧化活性。菌株的生长适应于胆盐条件，在胆盐平板试验中观察到明显的晕圈，并且在不同的消化轨迹条件下都能存活。Yd1对RAW 264.7细胞的作用是通过提高HO-1的水平来增加核因子-E2相关因子-2的转录和翻译活性。此外，与氧化应激组相比，YD1处理组显示出更高的抗氧化酶水平。YD1通过降低RAW 264.7细胞中一氧化氮(NO)和活性氧(ROS)的含量而显示出较强的抗氧化活性。本研究表明YD1菌株和该菌株可能分别是天然抗氧化剂和益生菌候选菌株。(C)2017爱思唯尔有限公司。保留所有权利。
A novel antioxidant peptide YD1 (similar to 1.0 kDa), purified from the strain Bacillus amyloliquefaciens CBSYD1, displayed activity in several in vitro assays and was also efficient against Gram-positive, Gram-negative as well as multidrug-resistant (MDR) bacteria. Strain growth was adapted to bile-salt conditions where a clear halos-zone was observed in a bile-salt plate assay and was viable in different digestive track conditions. YD1 treatment on RAW 264.7 cells increased the transcriptional and translational activities of NF-E2-related factor-2 (Nrf-2) through the enhanced levels of heme oxygenase-1 (HO-1). Furthermore, the YD1-treated group showed higher levels of antioxidant enzymes compared to the oxidative stress group. YD1 demonstrated a strong antioxidant activity by decreasing nitric oxide (NO) and reactive oxygen species (ROS) in RAW 264.7 cells. This study suggests that YD1 and the strain could be a natural antioxidant and a probiotic candidate respectively. (C) 2017 Elsevier Ltd. All rights reserved.