Cell death process induced by hydrogen peroxide is accelerated by clioquinol in rat thymocytes
Cell death process induced by hydrogen peroxide is accelerated by clioquinol in rat thymocytes
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发表时间：
2015-07
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通讯作者：
T. Oyama;E. Fukunaga;Y. Oyama
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作者：
T. Oyama;E. Fukunaga;Y. Oyama
我们用碘化丙啶和膜联蛋白V-FITC结合的流式细胞仪技术检测了CLOQOL对过氧化氢(H_2O_2)诱导的细胞死亡过程的影响，以了解CLOQOL是否增加了氧化应激引起的毒性。CLOQOL(100 NM)单独作用不能改变自发性细胞死亡的过程。但加速了300μM H_2O_2诱导的细胞死亡过程。结果表明，CLOQOL对H_2O_2诱导的细胞毒性有增强作用。因此，在治疗一些与氧化应激有关的疾病时，使用CLOQOL可能是不够的。
We examined the effect of clioquinol on the process of cell death induced by hydrogen peroxide (H2O2) using a flow cytometric technique with propidium iodide and annexin V-FITC in order to see if clioquinol augments the toxicity caused by oxidative stress. Clioquinol (100 nM) alone did not change the process of spontaneous cell death. However, the agent accelerated the process of cell death induced by 300 μM H2O2. Result indicates that clioquinol augments the cytotoxicity induced by H2O2. Therefore, the use of clioquinol may be inadequate for the treatment of some diseases related to oxidative stress.