Silk fibroin produced by transgenic silkworms overexpressing the Arg-Gly-Asp motif accelerates cutaneous wound healing in mice
Silk fibroin produced by transgenic silkworms overexpressing the Arg-Gly-Asp motif accelerates cutaneous wound healing in mice
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DOI：
10.1002/jbm.b.34098
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发表时间：
2019-01-01
影响因子：
3.4
通讯作者：
Kanekura, Takuro
中科院分区：
文献类型：
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作者：
Baba, Atsunori;Matsushita, Shigeto;Kanekura, Takuro
我们研究了丝素蛋白（SF）对小鼠伤口愈合的影响。将SF或由野生型家蚕（WT-SF，WT-ASFF）或过表达Arg-Gly-Asp（RGD）序列的转基因蠕虫（TG-SF，TG-ASFF）产生的丝制备的无定形SF膜（ASFF）放置在8-12周龄BALB/c小鼠背部通过活组织检查穿孔形成的5 mm直径全层皮肤伤口上。每个伤口用WT-ASFF和聚氨酯膜（UF）、TG-ASFF加UF或单独UF（对照）覆盖。在4、8和12天后分析伤口闭合、组织学厚度、肉芽组织面积和新血管形成。SF对细胞迁移和增殖的影响在体外通过划痕和MTT试验使用人真皮成纤维细胞进行了检查。TG-ASFF促进伤口闭合，ASFF治疗伤口的肉芽组织比对照组更厚更大，WT-ASFF显著促进新生血管形成。两种试验均显示SF诱导人真皮成纤维细胞的迁移和增殖。TG-ASFF和TG-SF对伤口闭合、肉芽形成和细胞增殖的影响比WT-ASFF和WT-SF更深刻。我们记录了SF加速皮肤伤口愈合，并且TG-SF增强了这种效果。(c)2018年威利期刊公司J Biomed Mater Res Part B：Appl Biomater，107 B：97-103，2019。
We investigated the effect of silk fibroin (SF) on wound healing in mice. SF or an amorphous SF film (ASFF) prepared from silk produced by the wild-type silkworm Bombyx mori (WT-SF, WT-ASFF) or by transgenic worms that overexpress the Arg-Gly-Asp (RGD) sequence (TG-SF, TG-ASFF) was placed on 5-mm diameter full-thickness skin wounds made by biopsy punch on the back of 8-12 week-old BALB/c mice. Each wound was covered with WT-ASFF and urethane film (UF), TG-ASFF plus UF, or UF alone (control). Wound closure, histological thickness, the area of granulation tissue, and neovascularization were analyzed 4, 8, and 12 days later. The effect of SF on cell migration and proliferation was examined in vitro by scratch- and MTT-assay using human dermal fibroblasts. Wound closure was prompted by TG-ASFF, granulation tissue was thicker and larger in ASFF-treated wounds than the control, and neovascularization was promoted significantly by WT-ASFF. Both assays showed that SF induced the migration and proliferation of human dermal fibroblasts. The effects of TG-ASFF and TG-SF on wound closure, granulation formation, and cell proliferation were more profound than that of WT-ASFF and WT-SF. We document that SF accelerates cutaneous wound healing, and this effect is enhanced with TG-SF. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 107B: 97-103, 2019.